白菜丝核菌叶腐病病原菌鉴定及生物学特性研究  被引量:9

Identification and Biological Characteristics of Pathogen of Chinese Cabbage Rhizoctonia Leaf Rot

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作  者:刘志恒[1] 李艳君[1] 杨红[1] 钱国东[1] 孙俊[1] 

机构地区:[1]沈阳农业大学植物保护学院,辽宁沈阳110161

出  处:《北方园艺》2009年第5期57-60,共4页Northern Horticulture

摘  要:对白菜丝核菌叶腐病标样进行了鉴定及生物学特性研究。结果表明:白菜丝核菌叶腐病病原为立枯丝核菌(Rhizoctonia solani Kühn)。病原菌菌丝最适生长条件为:20~25℃,pH 7,PDA培养基,黑暗条件,在供试的碳、氮源中,对可溶性淀粉和酵母浸膏利用最好;菌核形成的最适条件为:28℃,pH 7,查氏培养基,光照条件,以可溶性淀粉和蔗糖为碳源的查氏培养基有利于菌核的形成,硫酸铵培养基主要在气生菌丝上形成小而多的菌核,甘氨酸培养基形成的菌核较大。The results showed that the pathogen of Chinese cabbage Rhizoctonia leaf rot was Rhizoctonia solani. The optimum conditions for mycelia growth were that 20 -25℃, pH 7, PDA culture medium and darkness, Through carbon sources testing, soluble starch was found to support myeelium growing well. Among the nitrogen sources tested, yeast extract supported the fastest growth of mycelium; The optimum conditions for sclerotia generation were that 28℃, pH 7,Czapek culture medium and light; The beneficial carbon sources for the formation of sclerotia were soluble starch and sucrose; Small and more sclerotia were formed on the mycelium in the ammonium sulfate culture medium; The sclerotia which were formed in the glycine culture medium were larger.

关 键 词:白菜丝核菌叶腐病 立枯丝核菌 生物学特性 

分 类 号:S436.341.19[农业科学—农业昆虫与害虫防治]

 

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