食蟹猴经股静脉导管长期给药方法的建立  

Establishment of Chronic Administration by Femoral Venous Catheter in Cynomolgus Monkeys

作　　者：蒋星[1,2] 肖溢[1,2] 吕雷[2] 刘小波[2] 庞凌烟[1,2] 王莉[2] 

机构地区：[1]四川大学华西药学院,成都610041 [2]国家成都中药安全性评价中心

出　　处：《四川动物》2009年第3期444-447,共4页Sichuan Journal of Zoology

摘　　要：目的建立一种可靠的食蟹猴经股静脉导管长期给药的方法。方法选取20只雄性食蟹猴,麻醉后经股静脉造口,插入静脉导管,将植入式通道与导管连接并将其埋植于背部皮下,通过与植入式通道相连的输液泵进行给药。分别于术前1周、术后1周及术后2周测定血常规、血凝、生化等值。结果全部动物都能完成为期3个月的给药,手术感染率、导管堵塞率较低,分别为10%和5%。术后两周89.5%的生理指标与术前相比,差异无统计学意义(P>0.05)。结论该实验采用的食蟹猴经股静脉插管并通过输液泵进行长期给药是一种安全、有效的方法,可广泛用于药理学及毒理学实验。Objective To establish an animal model of chronic administration by femoral venous catheter in cynomolgus monkey. Method The femoral veins of twenty male eynomolgus monkeys were made stomas after anaesthesia. The vascular catheter connecting implanted access-port which was plugged in subcutaneous of subscapularis region was inserted into femoral vein. Administration was conducted by infusion pump connecting implanted access-port. Detection of hematology, blood coagulation , serum chemistry in cynomolgus monkeys were conducted on one week before operation, one week after operation and two weeks after operation respectively. Results A three-month period administration was conducted in all animals. The probability of infection and catheter obstruction were 10% and 5% respectively. 89.5% physiological indices had no significant difference between two weeks after operation and one week before operation. Conclusion Establishment of chronic administration by femoral venous catheter in eynomolgus monkeys is a safe and effective method. It can be widely used in pharmacological and toxicological experiments.

关 键 词：食蟹猴 股静脉导管 植入式通道 输液泵 

分 类 号：R96[医药卫生—药理学]