中国对虾抗病性状遗传标记筛选及遗传多样性分析  

Screen of white-spot-syndrome-virus(WSSV)-resistance molecular markers and analysis of genetic diversity in Fenneropenaeus chinensis

作　　者：李素红[1,2] 张天时[1] 孔杰[1] 孟宪红[1] 

机构地区：[1]农业部海洋渔业资源可持续利用重点开放实验室中国水产科学研究院黄海水产研究所,青岛266071 [2]青岛金海力水产科技有限公司,266071

出　　处：《渔业科学进展》2009年第2期54-59,共6页Progress in Fishery Sciences

基　　金：国家科技部项目(05EFN216900377);山东省自然科学基金项目(Y2004D05)共同资助

摘　　要：对人工选育的29个中国对虾半同胞家系进行人工感染WSSV后,选取存活时间最长和存活时间最短各29个个体,组成抗病群体和感病群体。用400个随机引物进行RAPD分析,得到与抗病正相关的特异性遗传标记5个,与抗病负相关的特异性遗传标记两个。15个随机引物在两个群体的扩增位点进行统计,共得到82个位点,其中多态位点34个,占41.46%,两个群体的多态位点比例分别为40.24%和37.8%。Shannon′s多样性指数估算两个群体的平均遗传多态度为0.2177,SLT群体的遗传多态度为0.2190略高于SST群体0.2163;群体内的遗传变异(HPOP/HSP)0.9645,可见96.45%的遗传变异来自于群体内,3.55%的变异来源于群体间。统计各座位的基因频率计算出两个群体的遗传分化指数为0.0294,表明两个群体并没有发生明显的遗传分化。29 sib-families of Fenneropenaeus chinensis which were obtained by selective breeding were artificially infected with WSSV. 29 samples with the longest survival time composed the WSSV-resistant group and 29 samples with the shortest survival time composed WSSV- susceptible group. Random amplified polymorphic DNA （RAPD） technique was employed to analyze these samples. The results were that 5 distinctive genetic markers with disease resistant trait and 2 distinctive genetic markers with disease susceptible trait were obtained. The genetic diversity of two groups was studied with 15 primers. 82 sites （DNA bands） were revealed, 34 of which were polymorphic, representing a percentage of 41.46 %. The proportions of polymorphic amplified fragments of the WSS-resistant group and the WSSV-susceptible group were 40.24% and 37.8%, respectively. The mean Shannon＇s index of genetic diversity in two groups was 0. 217 7. And the Shannon＇s index of the WSSV-resistant group was 0. 219 0, which was a little higher than that of the WSSV- susceptible group＇s 0. 2163. The average genetic variability of intra-polulation（HeoP/ Hsp）was 0. 964 5, indicating 96. 450/60 of genetic variability was from intra-polulation while 3.55 % was from inter-polulation. The genetic differentiation index analyzed with the gene frequency of each site was 0. 029 4 that indicated no genetic differentiation.

关 键 词：中国对虾 遗传标记 WSSV RAPD 

分 类 号：S945.1[农业科学—水产养殖] Q959.223.1[农业科学—水产科学]