慢性乙型肝炎共价闭合环状DNA定量测定在诊断和治疗中的价值  

Evaluation of the Value of Quantitative Assay of Hepatitis B Virus Covalently Closed Circular DNA in Diagnosis and Therapy of Chronic Hepatitis B

作　　者：马艳丽[1] 

机构地区：[1]附属菏泽市立医院,山东菏泽274000

出　　处：《菏泽医学专科学校学报》2009年第1期1-3,共3页Journal of Heze Medical College

基　　金：山东省自然科学基金课题项目;项目编号:Y2005C19

摘　　要：目的探讨荧光定量聚合酶链反应(FQ-PCR)检测慢性乙型肝炎(CHB)肝组织HBVcccDNA定量在CHB诊断和治疗中的价值。方法分别采用FQ-PCR、酶联免疫吸附分析法(ELISA)检测48例CHB和10例非CHB肝组织HBVcccDNA、肝组织和血清HBVDNA定量、乙型肝炎病毒标志物(HBVM)。同时用链霉菌抗生素蛋白-过氧化物酶连接法(SP)检测肝细胞中HBcAg表达。研究肝组织HBVcccDNA与HBVD-NA、HBeAg、肝细胞HBcAg水平及肝实质损害间的关系。结果(1)肝组织cccDNA定量与组织、血清HBVD-NA定量呈正相关(r=0.837,P<0.001;r=0.627,P<0.005)。(2)肝组织cccDNA定量与肝细胞内HBcAg半定量呈正相关(r=0.618,P<0.005)。(3)肝组织cccDNA定量与肝实质损害无明显相关(P>0.05)。(4)HBeAg阳性较抗-HBe阳性者肝组织cccDNA定量、肝组织和血清HBVDNA定量高(P<0.05)。结论FQ-PCR法检测肝组织cccDNA定量是评价HBV复制最直接可靠的指标,在CHB的诊断和治疗中有重要意义。Objective To evaluate the value of quanti.tation of HBVcccDNA by real- time polymerase chain reaction（PCR） in the diagnosis and treatment of chronic hepatitis B （CHB）. Methods Quantitative PCR were used to detect HBVcccDNA in liver biopsies. The same method was uased to examine HBVDNA in sera and liver biopsies. ELISA（ Enzyme- Linked Immunosorbent Assay） was used to detect hepatitis B serum markers. Expression of HBcAg in Liver tissue was detected by usingsP method. These measures were used to illuminate the relationships among the quantitation ofcccDNA, serume antigen quality, the quantity of HBV DNA, the quantity of HBcAg in hepatocytes, and liver damage. Results （ 1 ） There was a strong correlation between the cccDNA levels in the biopsy samples and HBVDNA levels in the sera and liver biopsies. （2） There was a significant correlation between cccDNA copy number and the number of HBcAg ＋ cells. （3）The correlation between quantitation of cccDNA in liver biopsies and histological activity index was not found. （4 ）HBeAg- positive patients had higher median total intrahepatic and sera HBVDNA （and intrahepatic cccDNA levels than anti- HBe - positive patients. Conclusions Quantitation of HBVcccDNA by real - time fluorescence PCR is a sensitive, stable, and reliable marker to assess HBV replication. And it plays a significant role in the diagnosis and treatment of CHB.

关 键 词：肝炎病毒 乙型 DNA 病毒 

分 类 号：R373.21[医药卫生—病原生物学] R512.62[医药卫生—基础医学]