反相高效液相色谱法测定抗骨松冲剂中淫羊藿苷的含量  

Determination of Icariin in Kanggusong Granula by RP-HPLC

作　　者：赵丁[1] 蒋晔[1] 刘伟娜[1] 张振华[1] 郑小丽 王秀云 

机构地区：[1]河北医科大学药学院,石家庄050017

出　　处：《中国现代应用药学》1998年第1期30-31,共2页Chinese Journal of Modern Applied Pharmacy

摘　　要：采用反相高效液相色谱法测定抗骨松冲剂中淫羊藿苷的含量。色谱柱Nucleosil C_(18)(5pm)200×4.6mm。流动相 甲醇—水—冰醋酸(60:40:0.5)。流速 1.0ml/min。检测波长268nm。内标 非那西丁。方法线性范围:0.0208—0.1040mg/ml。r=0.9998 平均回收率:101.20％。A RP-HPLC method was established for analysing icariin in kanggusong granula. Icariin was separat-ed on Nucleosil C18 column with a mobile phase of MeOH-H2OHAc(60:40:0. 5, v/v) and detected at 268nm, us-ing phenacetin as internal standard. The flow rate was 1.0 ml/min. The column temperature was 26℃　The linear range was 0. 0208-0.1040mg/ml, r=0.9998. The average recovery of icariin was 101.20% .

关 键 词：高效液相色谱 淫羊藿苷 抗骨松冲剂 

分 类 号：R286.0[医药卫生—中药学]