洋葱细胞骨架制备条件优化及影响因素研究  

Study on the Optimization of Preparation Condition for Cytoskeleton from Onion Inner-epidermal Cell and on its Influential Factors

作　　者：马云[1] 王启钊[1] 王伍[1] 柴翠翠[1] 

机构地区：[1]信阳师范学院生命科学学院,河南信阳464000

出　　处：《新乡学院学报》2009年第1期37-40,共4页Journal of Xinxiang University

基　　金：河南省自然科学基金项目(082300450490);河南省教育厅高等学校青年骨干教师资助计划;信阳师范学院青年骨干教师资助计划(2006BAD04A16)

摘　　要：以洋葱为材料,研究1%Triton X-100抽提非骨架蛋白时间、细胞环境温度和紫外线照射活细胞对洋葱细胞骨架形态观察效果的影响。结果表明:1%Triton X-100抽提非骨架蛋白25 min观察效果较好,细胞处在20℃和4℃环境下骨架清晰完整,呈网络状分布;-18℃、50℃及紫外线照射60 min处理均会造成细胞骨架不同程度的解聚,影响细胞骨架的观察。Cytoskeleton was an important construction of life cell. It was very senseful for investigating its function to observe the form of cytoskeleton. In order to study the crucial factor of this experiment, four different time（10min, 15min, 25min,40min） of extracting non-cytoskeleton protein was set to optimize microscopic observation of onion cytoskeleton. At the same time, influence factors of treating with different temperature（--18℃,4℃,20℃ ,50℃） and UV-B radiation（60min） were studied. Results showed that extracting non-cytoskeleton protein with 10% Triton X- 100 for 25min had a positive effect on the microscopic observation on cytoskeleton of onion inner--epidermal cell. Low temperature（-- 18℃） ,high temperature（50℃） and UV-B radiation （60min）had a negative effect on eytoskeleton.

关 键 词：细胞骨架 细胞环境温度 紫外线 洋葱鳞茎 显微观察 

分 类 号：Q245[生物学—细胞生物学]