血小板生成素基因注射促血小板生成作用的实验研究  

TPO gene injection promoted platelet production: a primary study

作　　者：赵建增 刘丽[1] 李奕 王艳华 梅英杰[1] 芦兴武 

机构地区：[1]空军总医院临床分子生物学研究中心

出　　处：《军医进修学院学报》1998年第1期40-42,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的：研究血小板生成素（ＴＰＯ）基因注射法对健康小鼠血小板的促生成作用。方法：将带有ｈＴＰＯｃＤ－ＮＡ的ｐｃＤＮＡ３质粒按６０μｇ／只剂量注射到１７５只昆明小鼠后肢内侧肌肉内，每天断尾采血计数白细胞和血小板，观察不同时间的骨髓和脾脏组织学变化。周期未注射小鼠做为对照组。结果：在基因注入后的３ｄ起即出现血小板和巨核细胞增多。在所观察的２７ｄ内，血小板有一可持续１周的高水平期，平均约为同期对照的１８４％，最高可达３倍以上。计数高峰后血小板在高于对照水平上呈波动状态。小鼠脾脏出现以巨细胞增生和内皮细胞活化为特点的组织学变化。结论：ＴＰＯ基因注射可刺激血小板和巨核细胞增殖，同时提示ＴＰＯ具有刺激免疫功能的作用。Objective:To investigate the effects of hTPO gene injection on mice platelet (PLT) production.Methods:hTPO cDNA cloned in pcDNA3, an eukarocytic expression plasmid, was administered into 175 mice skeleton muscle (60μg each). White blood cells (WBC) and PLTs in blood that collected by tail cut were counted, and the histology of bone marrow and spleen studied. Same aged uninjected mie were used as control. Results:Increase of megakaryocyte density and PLT count appeared on the second and third day respectively after injection. In about 4 weeks observation, PLT increase lasted for about 1 week, its average number was about 184% of the control, and the peak value reached over 3 times. Since then, PLT count fluctuated but it was still higher than the control. WBC counts were not significantly changed except the first 2 days. The histological features of the spleens showed macrophage proliferation and an immunological response.Conclusion:TPO gene injection strongly promotes platelet production and TPO is also probably an immune stimulator.

关 键 词：血小板生成素 基因注射 血小板生成 

分 类 号：R457.9[医药卫生—治疗学]