聚乙二醇渗透胁迫对藜幼苗叶片干旱诱导蛋白和可溶性蛋白的影响  

Effects of PEG Simulated Drought on Drought-induced Protein and Soluble Protein in the Leaves of Chenopodium album L.

作　　者：孙存华[1,2] 杜伟[1] 陈湘玲[1] 徐新娜[1] 张亚红[1] 孙东[1] 施剑杰[1] 

机构地区：[1]徐州师范大学生命科学学院,江苏徐州221116 [2]徐州师范大学江苏省药用植物生物技术重点实验室,江苏徐州221116

出　　处：《安徽农业科学》2009年第10期4373-4374,4408,共3页Journal of Anhui Agricultural Sciences

基　　金：徐州师范大学自然科学基金重点项目(06XLA11);徐州师范大学国家自然科学基金预研重点项目(08XLY06);国家林业科技支撑计划子专题项目(2006BAD03A00-6)资助

摘　　要：[目的]探讨渗透胁迫对藜幼苗叶片干旱诱导蛋白和可溶性蛋白的影响。[方法]用浓度5%、10%、20%聚乙二醇(PEG-6000)模拟干旱对藜幼苗进行胁迫处理,测定相关生理参数的变化。[结果]SDS-PAGE电泳结果显示,PEG胁迫可引起藜叶片产生110.3、103.9、22.7、19.2 kD 4种新的蛋白谱带,其中110.3、103.9 kD蛋白谱带的量随PEG浓度的增加而增加。随着胁迫时间的延长,浓度5%PEG胁迫下叶片中可溶性蛋白含量的变化表现为缓慢上升的趋势,浓度10%、20%PEG胁迫下叶片中可溶性蛋白含量的变化表现为先升后降的趋势。[结论]藜在渗透胁迫下,可溶性蛋白质增加,并有新的蛋白质合成。[Objective] The study aims to discuss the effect of osmotic stress with PEG on drought-induced protein and soluble protein in the leaves of Chenopodium album L..[Method] Seeding of Chenopodium album L.is treated with 5%,10%,20% polyethylene glycol（PEG-6000）for simulated drought inducement,changes of relative physiological parameters were determined.[Results]The results show that polypeptides with molecular weights of approximately 110.3,103.9,22.7 and 19.2 kD are induced from leaves on SDS-PAGE gel.Two protein bands of 110.3 and 103.9 kD are enhanced dramatically with the increasing of PEG-6000 concentrations.With the time prolonged,the soluble protein content increases slowly under 5% PEG stress,but under 10 % and 20 %PEG stress,the soluble protein content increases initially,and then decreased.[Conclusion] The soluble protein content is increasing and there is new protein in the leaves of Chenopodium album L.under osmotic stress.

关 键 词：藜 PEG模拟干旱 干旱诱导蛋白 可溶性蛋白 

分 类 号：S311[农业科学—作物栽培与耕作技术]