啤酒大麦幼胚愈伤组织诱导和植株高效再生  

Callus Induction and Efficient Plant Regeneration from Immature Embryos of Elite Barley Varieties

作　　者：李静雯[1] 张正英[1] 李淑洁[1] 赵丽娟[1] 

机构地区：[1]甘肃省农业科学院生物技术研究所,甘肃兰州730070

出　　处：《安徽农业科学》2009年第10期4375-4377,4470,共4页Journal of Anhui Agricultural Sciences

基　　金：甘肃省农牧厅生物技术专项"啤酒大麦籽粒蛋白质表达的遗传调控及种质创新"(GNSW-2006-05)

摘　　要：[目的]筛选出组织培养特性优良的大麦遗传转化受体。[方法]以7个大麦优良品种为实验材料,幼胚为外植体,研究基因型、培养基、激素等对大麦幼胚愈伤组织的诱导及植株再生的影响。[结果]在愈伤组织诱导过程中,甘啤4号等4个品种在三种诱导培养基上出愈率均较高,达100%;2,4-D与dicamba联用的MS2培养基上形成的愈伤组织质量优于MS1和MS3;品种间愈伤组织分化率差异显著,秀81-47分化率为88.9%,略低于模式品种Golden Prom ise;不同分化培养基的分化效率不同,在RN1培养基上的平均分化率较高。[结论]该试验筛选出愈伤组织诱导频率和绿苗分化率均较高,适合于遗传转化的受体材料,如秀81-47,甘啤4号。[Objective]To screen barley varieties suitable for genetic transformation that were excellent in tissue culture.[Method]Seven barley elite 1 ines and cultivars were studied to determine the influence of genotypes,media and plant hormones on the immature embryo derived callus induction and plant regeneration.[Result]The results indicated that the callus induction rate of four barley cultivars were very high on three different induction media.The quality of callus from MS2,which contained two plant hormones 2,4-D and Dicamba,were better than that from MS1 and MS3.Furthermore,differentiation frequencies among different varieties also varied significantly.Xiu 81-47 had a frequency of 88.9%,which was slightly less than Golden Promise.Two differentiation media had different effects on differentiation of barley varieties,and RN1 was better than RN2.genotypes.[Conclusion]Among the seven barley cultivars Xiu 81-47 and Ganpi 4 were identified with good performance in callus induction and plant regeneration.

关 键 词：大麦 幼胚 愈伤诱导 基因型 植株再生 

分 类 号：S512.3[农业科学—作物学]