柱杨的高效离体快繁技术  

Study on in vitro Propagation Techniques of Swedish Columnar Aspen

作　　者：刘玉冬[1] 刘艳军[1] 杨静慧[1] 朱文碧[1] 武春霞[1] 

机构地区：[1]天津农学院,天津300384

出　　处：《安徽农业科学》2009年第10期4378-4379,4381,共3页Journal of Anhui Agricultural Sciences

基　　金：北方耐盐耐旱柴油植物工程育种(08ZCKFNCO1200)

摘　　要：[目的]为利用组织培养技术培育柱杨新品种提供理论依据。[方法]以柱杨试管苗为材料,取其茎段为外植体,筛选最佳芽诱导培养基;分别以试管苗的叶片、茎段和叶柄为外植体,研究不同组织的再生芽诱导率;配制不同激素浓度的生根培养基诱导组培苗生根,筛选最佳生根培养基。[结果]培养基MS+2.0 mg/L6-BA+0.40 mg/LIAA诱导再生芽的效率最高,平均每块外植体再生芽8.6个,但芽的质量较差。综合考虑,较理想的芽诱导培养基为MS+1.0 mg/L6-BA+0.50 mg/L IAA;柱杨茎段产生再生芽的能力最强,叶柄次之,叶片最差;培养基1/2MS+0.2 mg/LNAA与1/2 MS+0.1 mg/LNAA诱导组培苗的生根率最高,每株平均生根数达8条以上,但根的质量较差。综合考虑,最佳生根培养基为1/2 MS+0.2 mg/LIAA。[结论]柱杨最佳芽诱导培养基为MS+1.0 mg/L6-BA+0.50 mg/LIAA,最佳生根培养基为1/2 MS+0.2 mg/LIAA。[Objective] The aim was to provide the theoretical basis for breeding new varieties of Swedish columnar aspen with tissue culture technology.[Method] With plantlet of Swedish columnar aspen as the material,its stem was taken as the explants to screen the optimal medium for bud induction With the leaf,stem and petiole of the plantlet as explants,the induction rate of regeneration buds of different tissues was investigated.The rooting mediums with different concn of hormone were prepared to induce rooting of plantlet and the optimal rooting medium was screened out.[Result] The induction efficiency of regeneration buds in medium of MS＋2.0 mg/L 6-BA＋0.40 mg/L IAA was the highest and the average bud number per explants was 8.6,but the quality of bud was worse.Comprehensive consideration,the optimal medium for bud induction was MS＋1.0 mg/L 6-BA＋0.50 mg/L IAA.The ability of producing regeneration buds of Swedish columnar aspen stem was the highest,the following was that of petiole and that of leaf was the poorest.The rooting rate of tissue culture seedling in mediums of 1/2 MS＋0.2 mg/L NAA and 1/2 MS＋0.1 mg/L NAA was the highest,the average root number per plant was more than 8,but the quality of root was worse.Comprehensive consideration,the optimal rooting medium was 1/2 MS＋0.2 mg/L IAA.[Conclusion] The optimal medium for buds induction of Swedish columnar was MS＋1.0 mg/L 6-BA＋0.50 mg/L and the optimal rooting medium was 1/2 MS＋0.2 mg/L IAA.

关 键 词：柱杨 培养基 激素浓度 

分 类 号：S722.37[农业科学—林木遗传育种]