银杏叶多糖抑制人白血病细胞增殖的试验研究  

Experimental Study on Inhibition of Polysaccharides of Ginkgo Biloba Leaf on Proliferation of Human Leukemia Cell

作　　者：张丽娇[1] 佟巨慧[2] 费瑞[2] 

机构地区：[1]长春师范学院生命科学学院,吉林长春130032 [2]吉林大学白求恩医学部,吉林长春130021

出　　处：《安徽农业科学》2009年第10期4501-4502,共2页Journal of Anhui Agricultural Sciences

基　　金：吉林省科技发展计划项目(200705354)

摘　　要：[目的]明确银杏叶多糖(PGBL)对白血病的治疗价值。[方法]以干燥银杏叶为材料,提取PGBL;向100μl对数增长期的人急性早幼粒白血病HL-60细胞悬液中分别加入100μl含PGBL50、100、200μg/ml的RPMI-1640培养液,以加入100μlRPMI-1640纯培养液为对照,研究不同浓度(50、100、200μg/ml)PGBL对HL-60细胞增殖的抑制率。[结果]50、100、200μg/ml PGBL对人急性早幼粒白血病HL-60细胞增殖均有不同程度的抑制作用,且随着药物浓度和作用时间的增加,抑制作用增强,即PGBL对HL-60细胞增殖的抑制作用存在剂量-时间依赖性;方差分析结果表明,PGBL对HL-60细胞增殖的抑制率显著高于对照(P<0.01)。[结论]PGBL对HL-60细胞增殖具有较好的抑制作用,且该抑制作用具有浓度-时间依赖性。[Objective] The aim was to determine the therapeutic value of polysaccharides of ginkgo biloba leaf（PGBL） on leukemia.[Method] With dry ginkgo biloba leaves as the materials,the PGBL was extracted from them.100 μl RPMI-1640 solution with 50,100,200 μg/ml PGBL was added into the suspension of human acute myeloid leukemia cell line HL-60 which in logarithmic growth period,with the treatment adding 100 μl RPMI-1640 pure solution as CK.The inhibition ratio of PGBL at different concn.（50,100,200 μg/ml） on proliferation of HL-60 cell was investigated.[Result] 50,100,200 μg/ml PGBL had different degree inhibition on proliferation of HL-60 and the inhibition was increased with the increase of drug concn.That was the inhibition of PGBL on proliferation of HL-60 cell had dose-time dependence.The results of variance analysis showed that the inhibition of PGBL on proliferation of HL-60 cell was significantly higher than that of CK（P〈0.01）.[Conclusion] PGBL had a good inhibition on proliferation of HL-60 cell and the inhibition had dose-time dependence.

关 键 词：银杏叶多糖 HL-60细胞 增殖 

分 类 号：S567.15[农业科学—中草药栽培]