归红注射液对肢体缺血-再灌注损伤的影响  

作　　者：范志英[1,2] 熊光仲[1] 袁峰[1] 朱付平[1] 

机构地区：[1]中南大学湘雅二医院,410011 [2]长沙市中医医院本部,410100

出　　处：《当代医学》2009年第12期147-149,共3页Contemporary Medicine

摘　　要：目的探讨归红注射液对缺血-再灌注肢体的保护作用。方法健康雄性SD大鼠56只,随机分为正常组、缺血再灌注组及药物干预组,缺血再灌注组和药物干预组又按再灌注后不同的时间点各分为2h、4h、8h组。建立肢体缺血再灌注模型后,检测血清SOD、MPO及TNF-a浓度,取后肢腓肠肌观察干湿重比值检查。结果缺血再灌注组各时间点血清SOD浓度较正常组下降,差异具有显著性,药物干预组各时间点SOD较缺血再灌注组高,有显著性差异("P<0.05");再灌注后血清MPO、血清TNF-a都升高,较正常组比较都有显著性差异("P<0.05"),而干预组各时间点各观测指标数值较缺血再灌注组低,差异具有显著性("P<0.05")。药物干预组各时间点骨骼肌干湿重比值较缺血再灌注各组低,两者比较都有显著性差异("P<0.05")。结论归红注射液能够减少氧自由基的产生,减少PMN的游出数量,降低血清中TNF-a的浓度及减轻组织水肿程度。Objective To investigate the protective effects of Carthamus tinctorius and Angelica sinensis injection on the limb ischemiareperfusion .Methods 56 healthy male S-D rats were randomly divides into control group, ischemia-reperfusion group and medicine-intervened group（which was injected Carthamus tinctorius and Angelica sinensis injection ）. Ischemia-reperfusion group and medicine-intervened group were divided into 2 hours, 4hours and 8hours group respectively according to the different rcperfusion time points. After the establishment of the limb ischemia-reperfusion rat model ,each group was observed by measuring the concentration of superoxide dismutase（SOD）, myeloperoxidase （MPO）, tumor necrosis factor-alpha（TNF-a）in the blood serum ,some posterior gastrocnemius musle was obtained to dry-humid weight ratio test.Result： The concentration of SOD in the blood serum in the ischemia-reperfusion group and in the medicine-intervened group was junior to it in the control group, Statistical analysis（one-way ANOVA） shows the level of the ischemia-reperfusion was lower than the control group（P〈0.05） and the level of the medicine intervention goup was lower than the ischemia reperfusion group at the different time point after reperfusion（P〈0.05） .The concentration ofMPO, TNF-a in the blood serum of the ischemia-reperfusion group were superior to that m the control group, Statistical analysis shows that there is significant difference in the level of those between the ischemia-reperfusion group and the control group（P〈0.05）. The concentration ofMPO, TNF-a in the blood serum of the medicine-intervened group were lower than that of the ischemia-reperfusion group, and Statistical analysis shows the differences between the medicine-intervened group and ischemia-reperfusion group are significant（P〈0.05）. The dry-humid weight ratio of the ischemia-reperfusion group were lower than that of the ischemia-reperfusion group, and Statistical analysis shows the differences between the medic

关 键 词：肢体缺血再灌注 归红注射液 药物治疗 血清 

分 类 号：R285.5[医药卫生—中药学] R978.19[医药卫生—中医学]