中国人群谷胱甘肽转移酶M1和T1的基因多态性分析：系统综述及吉林省结核涂阳人群研究  

Genetic polymorphism of glutathione-S-transferase M1 and T1： a systematic review in Chinese population and a pilot study in smear-positive pulmonary tuberculosis cases of Jilin province

作　　者：李晓婷[1] 袁燕莉[2] 夏惜惜[1] 于宝柱[2] 张铁娟[2] 刘欧[3] 吕晓珍[1] 詹思延[1] 

机构地区：[1]北京大学医学部公共卫生学院流行病与卫生统计学系,100191 [2]吉林省疾病预防控制中心 [3]北京大学医学部药学院

出　　处：《中华流行病学杂志》2009年第5期502-506,共5页Chinese Journal of Epidemiology

基　　金：国家自然科学基金（30771847）;中国全球基金第一轮结核病项目实施性研究（TB07-030）

摘　　要：目的了解谷胱甘肽转移酶M1（GSTM1）和T1（GSTT1）基因多态性在中国人群及吉林省结核涂阳人群中的分布。方法采用系统综述方法，以“GSTM1／GSTT1＋多态性”为关键词搜索国内发表于2009年1月以前、研究类型为横断面研究或队列研究基线的文献，经综合分析获得GSTM1、GSTT1基因多态性分布信息。以吉林省14个县（区）2007年11月至2008年5月间的全部结核涂阳病例（共1120名）为研究对象，采用多重PCR法检测GSTM1、GSTT1基因型。结果系统综述得到中国人群GSTM1、GSTT1基因纯合缺失型和GSTM1-GSTT1联合缺失基因型频率分别为54．2％、46．8％和26．2％，其中以汉族为主的人群分别为53．4％、44．9％和25．5％；本研究中吉林省结核涂阳人群相应频率分别为57．2％、20．4％和13．7％，GSTM1、GSTTI基因型及组合基因型分布的性别、年龄差异无统计学意义（P〉0．05）。与系统综述结果相比，本研究人群GSTM1纯合缺失基因型频率偏高（P=0．016），GSTT1纯合缺失基因型和GSTM1-GSTT1联合缺失基因型频率明显偏低（P值均〈0．001）。结论GSTM1、GSTT1基因多态性分布存在种族差异；本研究人群结果与系统综述结果的统计学差异可能是由于前者样本量较大、既往研究对象多为南方人群所致。Objective To investigate the distribution of glutathione-S-transferase M1 （GSTM1） and T1 （GSTT1） genes polymorphisms in Chinese population and smear-positive pulmonary tuberculosis cases of Jilin province. Methods Articles about GSTM1 and GSTT1 genes polymorphisms published before 2009 in China were searched. The study population was obtained from fourteen counties （or districts） of Jilin province, which included all cases from November, 2007 to May, 2008, totally 1120. The genotypes of GSTM1 and GSTT1 were detected by multiplex PCR technique. Results The frequencies of GSTM1 and GSTT1 ‘null＇ genotypes and combination M1-T1 ‘null＇ genotype acquired from systematic review were 54.2%, 46.8% and 26.2%, respectively, in Chinese Hans they were 53.4%, 44.9% and 25.5%, and in our research they are 57.2%, 20.4% and 13.7%, respectively. No significant differences between the frequencies of males and females as well as among that of different age groups were observed （P〉0.05）. The frequency of GSTM1 ‘null＇ genotype in our research is slightly higher than that in systematic review （P=0.016） , and the frequencies of GSTT1 ‘null＇ genotype and combination M1-T1 ‘null＇ genotype and are significantly lower than those in systematic review （both P〈 0.001）. Conclusion The frequencies of GSTM1 and GSTT1 ‘null＇ genotypes were different among ethnics. The statistical difference between systematic review and our research may due to our large sample size and mostly Southern people in previous studies.

关 键 词：谷胱甘肽转移酶 基因多态性 结核 系统综述 

分 类 号：R52[医药卫生—内科学]