土壤细菌对地黄试管苗和盆栽苗生长的影响  

Effects of inoculation with soil bacteria on growth of Rehmannia glutinosa in tube and potted plantlets

作　　者：王学翠[1,2] 温学森[1] 杨德奎[2] 

机构地区：[1]山东大学药学院,山东济南250012 [2]山东师范大学生命科学学院,山东济南250014

出　　处：《中草药》2009年第5期797-800,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30572326);国家"十一五"支撑计划项目(2006BA109B03)

摘　　要：目的探讨地黄连作障碍的原因和筛选可能具有缓解作用的有益菌。方法将已分离纯化的土壤细菌菌株接种到试管苗和盆栽苗基部,分别于30 d和60 d后收获,测量苗质量等指标。结果组培试验表明48个菌株中,有11个对地黄生长具有显著促进作用,另外11个具有抑制或致死作用;盆栽试验揭示16个菌株对地黄苗具有促进作用,13个菌株具有抑制或致死作用。结论土壤细菌对地黄苗的生长具有显著影响。地黄种植可能导致根际细菌菌群失衡,施用有益细菌可能是解决地黄连作障碍的有效措施。Objective To investigate the cause of continuous cropping obstacle of Rehrnannia glutinosa and screen beneficial bacteria. Methods In vitro cultured plantlets and potted plants were inoculated with different isolated soil bacteria. The plants were harvested and weighted in 30 and 60 d, respectively. Results In the in vitro culture experiment, 11 out of 48 strains displayed promoting action on the growth of plantlets, and 11 other strains showed inhibitory or lethal action. In the potted test, 16 strains showed promoting action and 13 strains showed suppressing or lethal action. Conclusion Soil bacteria influence the growth of R. glutinosa significantly. The flora of rhizosphere bacteria may be disturbed by the cultivation of R. glutinosa and inoculation of beneficial bacteria might be effective on the resolution of continuous cropping obstacle of R. glutinosa.

关 键 词：地黄 土壤细菌 连作障碍 有益细菌 

分 类 号：R282.2[医药卫生—中药学]