老年慢性肺心病患者外周血淋巴细胞凋亡的研究  

A study on apoptosis of peripheral blood lymphocytes in aged patients with chronic pulmonary heart-disease

作　　者：朱健[1,2] 唐靖渭[1,2] 李宁丽[1,2] 沈佰华 

机构地区：[1]上海第二医科大学附属第九人民医院 [2]上海免疫学研究所

出　　处：《中华老年医学杂志》1998年第1期23-25,共3页Chinese Journal of Geriatrics

基　　金：国家科学青年基金

摘　　要：目的研究老年慢性肺心病（ＣＰＨＤ）患者不同病期外周血淋巴细胞（ＰＢＬ）凋亡的变化，并探讨其临床意义。方法用ＤＮＡ断端标记法（ＴＤＴ法）测定ＰＢＬ在体外培养２４小时的凋亡发生率，并观察用脂多糖（ＬＰＳ）和金葡菌肠毒素Ｂ（ＳＥＢ）刺激ＰＢＬ后２４小时凋亡率的变化。结果患者急性加重期的ＰＢＬ体外培养２４小时后凋亡率（２９．９４％±１１．２４％）高于缓解期（１１．０４％±３．８６％）及健康组（９．２８％±２．５５％），加入ＬＰＳ及超抗原ＳＥＢ刺激后，缓解期患者的ＰＢＬ凋亡率明显增加，与健康组相比差异有显著性。结论老年ＣＰＨＤ患者ＰＢＬ凋亡率与病情有关，应用外源性刺激原可引起ＰＢＬ的激活诱导凋亡（ＡＩＣＤ）。Objective To study the apoptosis of peripheral blood lymphocytes (PBL) in aged patients with chronic pulmonary heart disease (CPHD). Methods In situ terminal deoxynuleotidyl transferasemediated nickend labeling (TDT) method was used. The PBL were isolated from the venous blood of the patients during different periods and cocultured with or without LPS or stephylococcal enterotoxin B (SEB) for 24 hours in vitro, then the cultured PBL were labelled by TDT method and identified quantitatively using FACScan. Results Apoptotic percent ages of the PBL in patients with acute exacerbation stage (2994±1124) were more than those of the PBL in controls (928±255) and remission stage (1104±386). When the PBL of both acute exacerbation and remission stage were cocultured with LPS or SEB, the percentages of apoptosis were higher than that of the PBL in controls population. Conclusions Apoptotic percent of PBL is related to the stage of aged patients with CPHD. LPS or SEB can induce activationinduced cell death (AICD) of PBL in vitro.

关 键 词：肺心病 淋巴细胞 细胞凋亡 老年人 

分 类 号：R541.503[医药卫生—心血管疾病]