醋柳黄酮对自发性高血压大鼠主动脉内MCP-1表达的影响  

Effect of Total Flavonoids of Hippophae Rhamnoides L.on the Expression of MCP-1 in Aorta of Spontaneously Hypertensive Rats

作　　者：何军[1] 陈仪[2] 肖恒怡[2] 常彬滨[1] 丁群芳[1] 章茂顺[3] 曾智[3] 张新军[1] 

机构地区：[1]四川大学华西医院老年科,成都610041 [2]四川大学华西医院老年医学实验室,成都610041 [3]四川大学华西医院心血管内科,成都610041

出　　处：《四川大学学报（医学版）》2009年第3期481-485,共5页Journal of Sichuan University(Medical Sciences)

基　　金：四川省科技厅攻关计划课题(05SG022-009)资助

摘　　要：目的研究醋柳黄酮(TFH)对自发性高血压大鼠(SHR)主动脉内单核细胞趋化蛋白-1(MCP-1)表达的影响,并探讨主动脉内MCP-1表达水平与主动脉内中膜厚度的关系。方法将12只12周龄雄性SHR大鼠随机分为3组,分别给予醋柳黄酮〔30mg/(kg.d),n=4〕、依那普利〔10mg/(kg.d),n=4〕、氢氯噻嗪〔25mg/(kg.d),n=4〕灌胃12周;另4只同周龄SHR和4只WKY大鼠予生理盐水灌胃12周,作为阳性对照和健康对照组。测量血压,检测主动脉内中膜厚度及管腔内径,ELISA检测血浆MCP-1浓度,real-ti me PCR检测主动脉MCP-1mRNA表达,免疫组化检测MCP-1水平。结果12周后,醋柳黄酮组血压明显降低(P<0.05),其疗效与依那普利组和氢氯噻嗪组没有差异;在降压疗效没有差异下,醋柳黄酮对主动脉内中膜增厚有明显的抑制作用(P<0.01),其作用与依那普利没有差别,强于氢氯噻嗪(P<0.01),且醋柳黄酮亦同时抑制主动脉内MCP-1mRNA和蛋白表达水平(P<0.01),其作用与依那普利没有差异,强于氢氯噻嗪(P<0.01)。结论醋柳黄酮可明显降低SHR大鼠血压,其疗效与依那普利和氢氯噻嗪没有差异。醋柳黄酮可独立于降压作用外抑制主动脉内MCP-1表达和抑制主动脉内中膜增厚,其效果与依那普利没有差别。醋柳黄酮可能通过抑制MCP-1达到抑制主动脉内中膜增厚、保护靶器官效果。Objective To study the effects of total flavonoids of Hippophae rhamnoides L. （TFH） on the expression of monocyte chemoattractant protein （MCP-1） in aorta of Spontaneously Hypertensive Rats （SHR） and to study the relationship of expression of MCP-1 in aorta and intimal medial thickness （IMT） of aorta. Methods Twelve-week-old male SHR （n= 12） were randomly devided into three groups to receive TFH （30 mg/（kg · d）, n =4], Enalapril [10 mg/（kg · d）, n= 4] and Hydrochlorothiazide [25 mg/（kg · d）,n= 4] for 12 weeks, respectively. Another 4 SHR and 4 WKY which receive placebo served as positive control group and negative control group. The systolic blood pressure （SBP）, intimal medial thickness and inside diameter of aorta of the rats were measured. The expression of MCP-1 in aorta was examined by real-time PCR and immunohistochemistry and the concentration of serum MCP-1 protein by ELSA. Results Twelve weeks later, systolic blood pressure in TFH was significantly decreased, compared with that in Enalapril and Hydrochlorothiazide without statistical differences. TFH markedly reduced the intimal medial thickness of aorta and expression of MCP-1 in aorta, similar with Enalapril, stronger than Hydrochlorothiazide. Conclusion TFH can markedly decrease SBP of SHR and the decrease value of the TFH group was similar with that of the Enalapril and Hydrochlorothiazide group. TFH may inhibit the expression of MCP-1 in aorta and intimal medial thickness of aorta beyond BP Lowering effect. The effect of THF on the expression of MCP-1 and intimal medial thickness of aorta was similar with Enalapril. TFH may through inhibite the expression of MCP-1 in aorta to reduce the intimal medial thickness of aorta and protect hypertensive injury in target organs.

关 键 词：醋柳黄酮 自发性高血压大鼠 内膜中膜厚度 单核细胞趋化蛋白-1 

分 类 号：R544.1[医药卫生—心血管疾病]