维生素E对老年雌性大鼠雌激素及海马芳香化酶表达的影响  

Effect of vitamin E on estrogen and expression of aromatase in hippocampus of senile female rats

作　　者：孔亚坤[1] 耿秀琴[1] 尹俊杰[1] 姚兰春[2] 周艳红[1] 郭献山[1] 

机构地区：[1]河南省新乡市中心医院,河南新乡453000 [2]哈尔滨医科大学生理学教研室

出　　处：《中国老年学杂志》2009年第9期1058-1060,共3页Chinese Journal of Gerontology

基　　金：黑龙江省自然科学基金资助项目(D200642)

摘　　要：目的探讨维生素E用于防治绝经后女性中枢神经系统退行性疾病的作用及机制。方法采用自然衰老雌性大鼠为动物模型,实验组每日注射维生素E5mg/kg(小剂量组)、15mg/kg(中剂量组)、60mg/kg(大剂量组)(n=8)。采用放免法检测血清雌激素含量,用免疫组化法观察维生素E对海马芳香化酶表达的影响,并与成年大鼠(对照组)对比观察。结果老年对照组较成年对照组雌激素水平下降,中、大剂量组血清雌激素水平回升(P<0.05),海马芳香化酶表达阳性细胞数目多、表达强,大剂量组呈强阳性。结论一定剂量维生素E不仅可以提高血中雌激素水平,而且可以通过增强海马芳香化酶的表达使局部雌激素浓度增加而发挥其神经保护作用。Objective To investigate the effect of vitamin E （Vit E） on estrogen and expression of aromatase in hippocampus of senile female rats and explore the effect of Vit E on preventing from postmenopausal female central nerve system degenerative diseases and its mechanism. Methods The animal model was established using senile female rats. Rats in low, middle and high dose of Vit E experimental groups （n=8） were respectively Vit E 5, 15 and 60 mg·kg-1·d-1. The levels of serum estrogen was detected by radioimmunoassay. The expression of aromatase in hippocampus was detected by immunohistochemistry. Results Compared with adult control group, the estrogen of elderly control group was low. And the estrogen of middle and high dose of Vit E groups were recovered （P〈0.05）, the expressions of aromatase in hippocampus from the two groups increased. The expression of aromatase in hippocampus in high dose of Vit E group showed strong positive. Conclusions Some dose of Vit E may not only elevate the level of serum estrogen, but strengthen the expression of aromatase in hippocampus, increase content of local estrogen and protect the nerve.

关 键 词：维生素E 自然衰老大鼠 雌激素 芳香化酶 神经保护 

分 类 号：R339.2[医药卫生—人体生理学]