狭叶柴胡种子萌发与内源抑制物质  

Seed Germination and Endogenous Inhibitory Substance of Bupleurum scorzoneraefolium

作　　者：周艳玲[1,2] 赵敏[1] 彭元举[1] 赵雨森[2] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040 [2]东北林业大学林学院,哈尔滨150040

出　　处：《植物研究》2009年第3期329-332,共4页Bulletin of Botanical Research

基　　金：黑龙江省自然科学基金(C00-33);黑龙江省重大攻关项目(GA02C401-02;GA06C30601);国家"十五"重大科技专项(2001BA701A59)

摘　　要：分别测定了柴胡种子的水浸提液、水浸泡液与乙醚浸提液对柴胡种子萌发以及对白菜和小麦种子萌发和幼苗生长的抑制活性;用水、乙醚、乙醇和丙酮浸泡柴胡种子,比较其对种子中抑制物质的去除效果;运用原位实验方法探讨柴胡种子萌发与内源抑制物质之间的关系。结果表明:柴胡种子中含有一定活性的内源抑制物质,其对白菜幼根生长的抑制活性高于对白菜种子萌发的抑制活性,对小麦叶绿素含量、鲜质量和α-淀粉酶的活性高于对小麦种子萌发的抑制活性,并且对柴胡自身种子萌发也有明显的抑制作用;室温用乙醚浸种24h可以提高种子发芽率。据此,建立了柴胡种子的催芽技术。An experiment was conducted to study the inhibitory activities of aqueous extracts, soaking soluti with water and extraction solution with aether from Bupleurum scorzoneraefolium seeds on seed germination on of B. scorzoneraefolium, seed germination and seedling growth of Brassica chinesis and wheat. The B. scorzoneraefolium seeds were soaked with water, aether, ethanol and acetone, respectively, and their effects on reducing inhibitory substance were compared. The effect of endogenous inhibitory substance on seed germination was studied in suit. Results showed that,the endogenous inhibitory substance existed in B. scorzoneraefolium seeds, and the inhibitory activity of endogenous inhibitory substance on the growth of young roots is stronger than that on seed germination of B. chinesis. The inhibitory activities of the endogenous inhibitory substance on the chlorophyll content, fresh weight and the activity of α-amylase are higher than those on seed germination of wheat. The endogenous inhibitory substance also significantly inhibits the seed germination of B. scorzoneraefolium. The germination percentage increases by soaking the seeds with aether for 24 hours under room temperature. A stratification technique for B. scorzoneraefolium seeds is hereby obtained.

关 键 词：柴胡 种子萌发 内源抑制物质 

分 类 号：S567.239[农业科学—中草药栽培]