白桦肌动蛋白(Actin)基因全长cDNA克隆与序列分析  

Cloning and Sequence Analysis of Full-length cDNA of Actin Gene from Birch(Betula platyphylla Suk.)

作　　者：陈鹏飞[1] 刘雪梅[1,2] 宋福南[2] 宋兴舜[1,2] 刘霓[1] 金微微[1] 刘威[1] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040 [2]东北林业大学林木遗传育种与生物技术教育部重点实验室,哈尔滨150040

出　　处：《植物研究》2009年第3期339-345,共7页Bulletin of Botanical Research

基　　金：黑龙江省"十一五"科技攻关项目(No.GA06B301-2-3);黑龙江省教育厅科技项目(No.11523003);哈尔滨市科技创新人才研究专项资金项目(2008RFQXN009)

摘　　要：以白桦(Betulaplatyphylla Suk.)次生木质部为材料,用改良CTAB方法提取总RNA。根据植物肌动蛋白(Actin)基因编码区的保守序列设计引物后进行RT-PCR,并采用RACE技术扩增出Actin基因全长序列。该基因cDNA全长1785bp,序列分析表明,该基因编码区1134bp,编码377个氨基酸,5′非编码区157bp,3′非编码区495bp。所得序列与GenBank中注册的其它植物肌动蛋白核苷酸序列的相似性均在80%以上,氨基酸序列的相似性高达96%以上。此基因已在GenBank注册(EU588981)。根据高等植物肌动蛋白相似性构建了进化树,表明白桦肌动蛋白与蓖麻肌动蛋白之间的亲缘关系最为密切,在进化中分化时间最为接近。Total RNA was isolated from Betula platyphylla xylem by the method of modified CTAB which was more effective and simple. The primers of plant actins conserved coding region were designed and a full-length cDNA of actin was obtained using RT-PCR and RACE techniques. Its full length is 1 785 bp. The sequence analysis result revealed that it contained a coding region of 1 134 bp encoding a protein of 378 amino acids, a 5＇- UTR of 157 bp and a 3＇-UTR of 495 bp. Homologous alignment shows that it shares over 80% nucleotide sequence similarity and over 96% amino acid sequence similarity with actins in other plants. The full length cDNA sequence of actin was registered in GenBank with accession number EU588981. The phylogenetic tree reconstructed on the base of amino acid sequences suggests that the relationship of actin between B. platyphylla and Ricinus communis is most intimate and they might have the same differential time in evolution.

关 键 词：白桦 肌动蛋白 基因克隆 序列分析 进化 

分 类 号：Q785[生物学—分子生物学]