紫外线致弱日本血吸虫尾蚴诱导BALB/c小鼠免疫保护作用研究  

The immuno-protective effect induced in BALB/c mice by the UV-attenuated cercariae of Schistosoma japonicum

作　　者：周霞[1] 诸葛洪祥[1] 龚唯[1] 骆伟[1] 魏莉[1] 杜海林[1] 陈明中[1] 

机构地区：[1]苏州大学医学部基础医学与生物科学学院病原生物学系,苏州215123

出　　处：《中国人兽共患病学报》2009年第5期446-448,482,共4页Chinese Journal of Zoonoses

摘　　要：目的观察紫外线(UV)致弱日本血吸虫尾蚴免疫BALB/c小鼠免疫保护作用。方法辐照致弱日本血吸虫尾蚴,经腹部皮肤用UV致弱日本血吸虫尾蚴免疫诱导BALB/c小鼠,3周后进行攻击感染,同时设正常感染对照组。攻击感染6周后解剖小鼠,计算减虫率、减卵率,并观察虫体发育情况和肝组织细胞免疫应答情况。结果免疫组的减虫率和减卵率分别为37.90%和51.16%;免疫组小鼠体内虫体发育明显滞后于正常感染对照组内虫体;免疫组小鼠肝脏虫卵肉芽肿较正常组明显减少。结论UV致弱日本血吸虫尾蚴免疫BALB/c小鼠能诱导较好的免疫保护力。To investigate the immuno protective effect induced in BALB/c mice by the UV-attenuated cercariae of Schistosoma japonicum, mice were immunized through abdominal skin, challenged 3 weeks post-immunization and were killed 6 weeks after challenge to examine the changes and to count the worm and egg reduction rates as well as to observe the developmental conditions of worms and the cell-mediated immune responses induced in liver tissues of the immunized mice. Control mice without immunization were set up for comparison. It was found that the worm and egg reduction rates of the immunized mice 37.9% and 51.16% respectively, and their developmental condition of worms was markedly delayed and less granulomatous response in liver induced by eggs was observed in comparison with the control mice. It is evident that the UV-attenuated S. japonicum cercariae can induce a middle level of immuno-protective effect in the immunized BALB/c mice.

关 键 词：日本血吸虫 BALB/C小鼠 紫外线致弱尾蚴 免疫病理反应 

分 类 号：R383.2[医药卫生—医学寄生虫学]