慢性盐负荷下自发性高血压大鼠体内氧化应激水平与内皮功能变化  

Effects of Chronic Salt Loading on Oxidative Stress and Endothelial Dysfunction in Spontaneously Hypertensive Rats

作　　者：刘富强[1] 牟建军[1] 卫娜[1] 吴冠吉[1] 蒋伟[1] 

机构地区：[1]西安交通大学医学院第一附属医院心内科,陕西西安710061

出　　处：《中华高血压杂志》2009年第5期440-443,共4页Chinese Journal of Hypertension

基　　金：国家自然科学基金资助项目(30671160)

摘　　要：背景盐是心血管疾病的重要危险因素之一,其作用机制可能与氧化应激和内皮功能失调有关。目的观察慢性高盐负荷对自发性高血压大鼠(SHR)血压、氧化应激水平及内皮功能的影响。方法5周龄雄性SHR48只,高盐组(4%NaCl)、正常摄盐组(0.4%NaCl)各24只,分别饲养4、8、12、16周处死(每次每组6只),取血清测定亚硝酸盐(NOx)、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性;取胸主动脉采用免疫组织化学方法观察内皮型一氧化氮合酶(eNOS)的改变。结果在实验各阶段,高盐组的收缩压显著高于正常摄盐组[4周(158.0±13.6)比(145.0±10.9)、8周(175.0±18.5)比(149.0±8.1)、12周(178.0±14.8)比(161.0±15.3)、16周(178.0±17.1)比(159.0±17.1)mmHg,P<0.05];高盐组的NOx(P<0.01)、MDA水平(P<0.05),以及eNOS表达水平(P<0.01)均高于正常摄盐组,且随着年龄时间呈明显上升趋势;两组血清SOD水平未发现明显差异(P>0.05)。结论高盐摄入可导致体内氧化应激水平升高和内皮功能失调,这可能是其增加心血管病发病风险的机制之一。Background High salt intake increase the risk of cardiovascular disease, the mechanis by which may be mediated by oxidative stress and endothelial dysfunction. Objective To investigate the change of oxidative stress and endothelial dysfunction in spontaneously hypertensive rats （SHR） during long term chronic salt loading. Methods Forty-eight male SHR （5 week old） were randomly to receive high salt （4% NaCl, n=24） or normal salt （0.40% NaCl, n=24）. Six rats were sacrificed every 4 weeks and the aortae were obtained to measure the eNOS expression by immunohistochemistry. Serum samples were collected to measure the concentration of serum super oxide dismutase （SOD）, methane dicarboxylic aldehyde （MDA） and nitric oxide metabolites （NOx）. Results Compared with normal salt group, the systolic blood pressure was significantly higher than that in normal salt group [4 weeks （158.0±13.6） vs （145.0±10.9） ; 8 weeks （175.0±18.5） vs （149.0±8. 1） ; 12 weeks （178.0±14.8） vs （161.0±15.3）; 16 weeks （178.0±17. 1） vs （159.0±17. 1）mmHg, P〈0. 05]. Concomitantly, the levels of serum NOx（P〈0.01）, MDA（P〈0.05） and eNOS （P〈0.01） were also higher. There were nomsignifantly increasing tendency of the increment of oxidative stress products with aging. Conclusion Salt increase the risk of cardiovascular disease associaled with increasing oxidative stress and nitric oxide generation.

关 键 词：盐 氧化应激 亚硝酸盐 一氧化氮合酶 自发性高血压大鼠 

分 类 号：R544.1[医药卫生—心血管疾病] R749.16[医药卫生—内科学]