吸入高浓度氧对大鼠脑组织匀浆内活性氧的影响  

The Effect by of Inspiring High Concentration Oxygen on the Reactive Oxygen Species in the Rats Brain Homogenates

作　　者：马亿选 潘晓军[2] 周海 朱文龙 

机构地区：[1]山东省电力中心医院麻醉科,济南250001 [2]山东大学齐鲁医院麻醉科 [3]徐州市人民医院麻醉科

出　　处：《医学研究杂志》2009年第5期99-101,共3页Journal of Medical Research

摘　　要：目的大鼠吸入一定时间的高浓度氧后,测定其脑组织匀浆内活性氧含量的变化,评价吸入高浓度氧的安全性。方法选用纯种wister大鼠60只,随机分为A、B、C、D、E5组,每组12只,其中A、B、C、D组为高氧组,分别放置于氧浓度为85%～100%的自制氧箱中,于实验4、8、12、16h后制取标本,E组为空气对照组。取脑组织低温高速粉碎、离心提取匀浆液,比色法测定脑组织匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)、一氧化氮合酶(NOS)、谷胱甘肽(GSH)、过氧化氢酶(CAT)和总抗氧化能力(T-AOC)的浓度。结果与对照组相比,高氧各组脑组织匀浆中MDA、NO、NOS均显著升高(P<0.01?,并随吸氧时间的延长有升高的趋势;而SOD、GSH、CAT、T-AOC均显著降低(P<0.01?,并随吸氧时间的延长有降低的趋势。结论吸入高浓度氧导致脑内活性氧浓度的升高,应引起重视。Objective To evaluate the security of inspiring high concentration oxygen by detecting the reactive oxygen species of the rats brain homogenates after inspiring high concentration oxygen for a certain time. Methods Sixty wister rats weighting 150 - 200g were randomly devided into five groups（ the group of 4h.8h,12h,16h and the control group,12/group）. The experimental rats were exposed to 85% - 100% oxygen in a self - made chamber and the control animals were kept in normal room air. The Concentration of the MDA, SOD, GSH, NO,NOS,CAT and T - AOC of the brain homogenates was detected. Results Compared with the control group, the level of MDA, NO and NOS in the brain homogenates grew up markedly（P 〈0.01 ） and SOD,GSH,CAT,T - AOC fell down accordingly（P 〈0.01 ）. Conclusion Inspiring high concentration oxygen can make the reactive oxygen species grown up, and we should pay attention to the resuit.

关 键 词：高浓度氧 脑组织匀浆 活性氧 

分 类 号：R285.5[医药卫生—中药学] R651.15[医药卫生—中医学]