胞外钙及胞内钙库钙在膀胱ICCs细胞自发性钙瞬变中的作用  

Role of Ca^(2+) entry and Ca^(2+) store in ICCs' spontaneous Ca^(2+) transients in rat bladder

作　　者：郑霁[1] 贾卓敏[1] 张永革[1] 潘进洪[1] 沈文浩[1] 李为兵[1] 金锡御[1] 宋波[1] 

机构地区：[1]第三军医大学西南医院全军泌尿外科研究所,重庆400038

出　　处：《第三军医大学学报》2009年第10期918-921,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30672093;30670776)~~

摘　　要：目的观察离体活膀胱肌条铺片中卡哈尔间质细胞(interstitial cells of cajal,ICCs)自发性钙瞬变,并检测胞外钙及胞内钙库钙对ICCs细胞自发性钙瞬变的影响,探讨ICCs细胞自发性钙瞬变产生的机制。方法制作离体膀胱肌条铺片,生理液灌流保持肌条铺片活性。待肌条自发性收缩活动出现后,Fluo-4负载肌条铺片,激光共聚焦显微镜观察其中ICCs细胞自发性钙瞬变,随后观察尼莫地平(Nimodipine)、2-氨基乙基二苯硼酸盐(2-aminoethoxydip henylborate,2-APB)、雷诺丁(Ryanodine)、毒胡萝卜素(Thapsigargin)及无钙、高钙生理液对ICCs细胞自发性钙瞬变的影响,探讨ICCs细胞自发性钙瞬变产生的机制。结果可见离体活膀胱肌条铺片中平滑肌细胞和ICCs细胞均产生节律性自发性钙瞬变,平滑肌细胞钙瞬变频率较快,而ICCs细胞钙瞬变频率相对较慢。尼莫地平能完全抑制平滑肌细胞的自发性钙瞬变,但对ICCs细胞的自发性钙瞬变无显著影响。2-APB、雷诺丁、毒胡萝卜素及无钙生理液均可完全抑制ICCs细胞的自发性钙瞬变,高钙生理液可显著增加ICCs细胞自发性钙瞬变的频率。结论细胞外钙离子通过非L型钙离子通道入胞及细胞内钙库放钙均参与ICCs细胞自发性钙瞬变的产生。Objective To observe the spontaneous Ca^2＋ transients of interstitial cells of cajal （ICCs） and explore its mechanism in the rat bladder. Methods Single layer of smooth muscle bundles attached to the serosal layer of rat bladder were prepared and superfused with warmed physiological saline （PSS） to recover and maintain their activity. After spontaneous muscle movements of the muscle layers were visually detected, the tissues were loaded with the fluo-4 AM and then spontaneous Ca^2＋ transients of ICCs in the muscle layers were observed with laser scanning confoeal microscopy. Nimodipine, 2-aminoethoxydiphenylborate （2-APB）, ryanodine, thapsigargin, Ca^2＋ free PSS and high Ca^2＋ PSS were then used to investigate the mechanisms of spontaneous Ca^2＋ transients in ICCs. Results Spontaneous Ca^2＋ transients of smooth muscle cells （SMCs） and ICCs in the smooth layers were successfully detected. Nimodipine suppressed significantly Ca^2＋ transients in SMCs but not in ICCs. Spontaneous Ca^2＋ transients of ICCs were completely abolished by 2-aminoethoxydiphenylborate （2-APB）, ryanodine, thapsigargin, Ca^2＋ free PSS and partly increased by high Ca^2＋ PSS. Conclusion These results suggest that spontaneous Ca^2＋ transients of ICCs rely on both Ca^2＋ release from intracellular Ca^2＋ stores and extracellular Ca^2＋ entry through non L type Ca^2＋ channels.

关 键 词：ICCS细胞 自发性钙瞬变 膀胱 

分 类 号：R322.62[医药卫生—人体解剖和组织胚胎学] R329.27[医药卫生—基础医学]