白细胞介素-17在原发性干燥综合征患者唇腺组织和外周血的表达  

Interleukin-17 expression in salivary glands and peripheral blood of patients with primary Sjogren＇s syndrome

作　　者：王喜梅[1] 厉小梅[1] 李向培[1] 王晓秋[2] 马艳[1] 王玮[1] 王怡平[3] 

机构地区：[1]安徽医科大学附属省立医院风湿免疫科,合肥230001 [2]安徽医科大学附属省立医院病理科,合肥230001 [3]澳大利亚悉尼大学Westmead医院肾脏移植中心

出　　处：《中华风湿病学杂志》2009年第5期292-295,I0001,共5页Chinese Journal of Rheumatology

基　　金：国家自然科学基金（30872340）;安徽省国际科技合作计划项目（08080703027）;中华医学会临床医学科研专项资金（08010200098）

摘　　要：目的探讨白细胞介素（IL）-17在原发性干燥综合征（pSS）患者唇腺组织及外周血及唾液中的表达及意义。方法采用免疫组织化学的方法对30例pSS患者及5名对照组的唇腺石蜡组织的连续切片进行IL-17、CD45RO和CD20染色。用双抗体夹心酶联免疫吸附试验（ELISA）法检测30例pSS患者和20名健康对照组外周血及唾液中IL-17水平。结果pSS患者唇腺组织均有IL—17阳性细胞表达；而对照组无表达。在pSS患者唇腺组织少量淋巴细胞浸润组，IL－17阳性细胞数（15±5）个低于多量淋巴细胞浸润组（21±8）个（P〈0．05）；但在少量淋巴细胞浸润的pSS组，IL－17阳性的淋巴细胞占总淋巴细胞的比率（14±5）％，高于多量淋巴细胞浸润组（10±4）％（P〈0．05）。在大量淋巴细胞聚集的部位，IL-17主要表达在腺体外周的淋巴细胞上，呈胞质表达。相关性分析显示：IL-17阳性细胞数与红细胞沉降率（ESR）呈显著正相关（r=0．557，P〈0．05）。血清中IL-17的浓度在多量淋巴细胞浸润组（7．8±0．6）pg／ml与少量淋巴细胞浸润组（7．6±1．0）pg／ml及健康对照组（7．4±1．0）pg/ml相比差异无统计学意义（P〉0．05）。结论IL-17在pSS患者中可能参与局部的炎症损伤过程。Objective To investigate the expression of interleukin-17 in the salivary gland and peripheral blood of patients with primary Sjogren＇s syndrome （pSS）. Methods Biopsy specimens of labial glands were collected from 30 patients and 5 controls. Immunohistochemistry examination was conducted to detect IL-17 expression cells （Th-17）, while CD45RO and CD20 were tested by using monoclonal antibodies. IL-17 levels were assessed by ELISA in 30 pSS patients and 20 healthy controls. Results Th-17 cell numbers in the salivary glands of pSS patients with few infiltrating lymphocytes were lower than that with massive infiltrating lymphocytes （15±5 vs 21±8, P〈0.05）. However, the percentage of Th-17 positive cells over the total infiltrating lymphocytes in the salivary glands of pSS patients with few infiltrating lymphcytes was higher than that with massive infiltrating lymphocytes （P〈0.05）. There was no expression of IL-17 in the controls. The distribution pattern of Th-17 was shown that Th-17 cells were distributed evenly in the salivary glands of pSS patients with few infiltrating lymphocytes, but only appeared in the periphery zone of massive or focal infiltration in the glands of pSS patients with massive infiltrating lymphocytes. The amount of Th-17 positive cells were associated with the disease activity which was evaluated by levels of erythrocyte sedimentation rate （r=0.557, P〈0.05）. There was no significant differ-ence in IL-17 levels of peripheral blood between patients with pSS and normal controls. Conclusion The results of this study have shown that Th-17 is expressed in the salivary glands of patients with pSS, which indicates that IL-17 may play an important role in the patho-genesis of salivary gland destruction.

关 键 词：干燥综合征 白细胞介素-17 涎腺 

分 类 号：R686[医药卫生—骨科学]