克山病病区玉米及酱块对大鼠心肌损伤的实验研究  

Experimental study on cardiac pathological change in rats fed with corn and bean puree of Keshan disease area

作　　者：张立君[1] 刘明法[1] 陈洁[1] 李少臣[1] 裴俊瑞[1] 周令望[1] 刘阳[1] 王铜[1] 于维汉[1] 梯保雄[1] 

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地手病控制中心克山病防治研究所,150081

出　　处：《中国地方病学杂志》2009年第3期291-293,共3页Chinese Jouranl of Endemiology

基　　金：基金项目：黑龙江省卫生厅课题（2006-286）

摘　　要：目的探讨克山病病区玉米及酱块与大鼠心肌损伤的关系。方法将雄性Wistar大鼠按体质量随机分为3组，分别饲以病区玉米、病区玉米＋酱块、非病区玉米，l、3个月时检测大鼠尾静脉全血谷胱甘肽过氧化物酶（GSH—Px）活力，6个月时光镜下观察大鼠心肌病理改变。结果1、3个月时3组大鼠全血GSH～Px活力比较，差异有统计学意义（F=23．60、72．46，P均〈0．01），其中病区玉米组[（181．58±22．15）、（44，76±28．59）U／L]和病区玉米＋酱块组[（195．03±17．66）、（30．38±3．35）U／L]明显低于非病区玉米组[（340．90±95．42）、（125．17±13．64）U／L，P均〈0．01]，而病区玉米组与病区玉米＋酱块组比较，未见明显改变（P〉0．05）。光镜下3组大鼠心肌损伤病理改变发生率分别为3／9、1／9、2／7，组间比较差异无统计学意义（P〉0．05）。结论．单纯克山病病区玉米饲养可引起大鼠心肌损伤病理性改变，酱块无加重心肌损伤作用。Objective To investigate the myocardial damage in rats fed with corn from Keshan disease area added with bean puree. Methods Male Wistar rats were randomly divided into 3 groups according to their body weights, and fed with corn, corn from Keshan disease area added with bean puree, corn from non-endemic area. The GSH-Px activity of vena cardalis blood was examined in I and 3 months, rats were sacrificed after being fed for 6 months to examine the heart changes with HE stain. Results The three groups of GSH-Px activity were different in I and 3 months respectively（F = 23.60,72.46, all P 〈 0.01 ） ; GSH-Px activity was （181.58 ± 22.15）, （44.76 ± 28.59）U/L in rats fed with corn, was （195.03 ±17.66）, （30.38 ± 3.35）U/L in those fed with corn added with bean puree from Keshan disease area, lower than the group fed with corn of non-endemic areal （340.90 ± 95.42）, （125.17 ± 13.64）U/L, all P 〈 0.01]. But the difference of GSH-Px activity between simple corn group and corn adding bean puree groups of Keshan disease area was not obvious（P 〉 0.05）. Myocardial damage incidence of the three groups was 3/9,1/9,2/7. Difference among three groups did not have statistical significance（X2= 1.33, P 〉 0.05）. Conclusions Only corn from Keshan disease area may induce myocardial damage pathology change. Adding bean puree into corn does not increase damage.

关 键 词：克山病 谷胱甘肽过氧化物酶 心肌 玉米 

分 类 号：R686[医药卫生—骨科学]