特纳10-AU现场荧光仪吸收光谱测定套件的研发及应用  

Development and Application of the Absorption Measurement Kit of 10-AU Field Fluorometer

作　　者：吴芳[1] 陈佳宁[1] 张勇[1] 

机构地区：[1]近海海洋环境科学国家重点实验室(厦门大学)/厦门大学环境科学研究中心,厦门361005

出　　处：《现代科学仪器》2009年第2期12-14,共3页Modern Scientific Instruments

基　　金：NSFC(20777062);SRFDP(200803840015);厦门大学科技创新项目(2)科学仪器研制与开发(XDKJCX20061022)资助。

摘　　要：针对天然水体中溶解有机物吸收和荧光无法同时测定的问题,对特纳10-AU现场荧光仪进行了改进。通过对仪器样品室的改造,利用反射镜改变光路,设置衰减片和降低光电倍增管电压控制光通量,在不破坏原有仪器结构下实现其吸收光谱测定功能。改进后的仪器测定重铬酸钾和高锰酸钾水溶液的灵敏度较Cary 100紫外可见分光光度计分别高约1倍和0.5倍,相对标准偏差分别为3.93%、2.12%(n=7)。实现了腐殖酸水溶液的吸收和荧光的同时测定,检出限分别为0.306mg/L和0.0104mg/L。实验证明改进后的10-AU现场荧光仪可用于水体中溶解有机物吸收光谱的测定,操作简便,测定结果可靠。A Turner 10 - AU field fluorometer was improved to solve problems of the study on simultaneously determination of dissolved organic matter absorption and fluorescent properties in natural water samples. The sample cell and cell holder of the fluorometer were modified with a mirror to develop a new absorption measurement kit, and attenuator plates and photomultiplier voltage were used to control the intensity of absorption light. The ab- sorption measurements of potassium dichromate and potassium permanganate solution were realized by the improved fluorometer. The Sensitivities of the improved fluorometer were about 1 and 0.5 times higher than that of Cary 100 UV -Vis spectrophotometer, with the RSD were 3.93% ,2.12% （n = 7）, respectively. Satisfactory results were both obtained for the absorption spectra measurement and the fuorescent determination of humic acid in water, detection limit was 0. 306mg/L and 0. 0104mg/L respectively. It was proved by experimental results that the improved fluorometer was successfully used to determine dissolved organic matter in water with simpler procedure and satisfactory results.

关 键 词：现场荧光仪 吸收光谱测定 重铬酸钾 高锰酸钾 

分 类 号：TH744.1[机械工程—光学工程]