大黄素对大鼠胰腺星状细胞TGF-β1含量的影响  

Effect of emodin on transforming growth factor-β1 in rat pancreatic stellate cells

作　　者：梁晓强[1] 章学林[2] 顾宏刚[2] 张静喆[2] 

机构地区：[1]上海中医药大学附属龙华医院中医外科研究所,上海市200032 [2]上海中医药大学附属龙华医院普外科,上海市200032

出　　处：《世界华人消化杂志》2009年第10期1018-1020,共3页World Chinese Journal of Digestology

基　　金：上海市重点学科基金资助项目;No.T0304;上海市医学领军人才基金资助项目;No.LJ06048;上海市教育委员会科研创新基金资助项目;No.09YZ131~~

摘　　要：目的:观察大黄素对大鼠胰腺星状细胞TGF-β1含量的影响.方法:取SD雄性大鼠胰腺进行胰腺星状细胞分离、鉴定及体外培养,传代后用不同浓度大黄素(20、40、60μmol/L)对其进行干预,用ELASA法观察胰腺星状细胞内TGF-β1含量的变化.结果:不同浓度大黄素TGF-β1含量明显低于对照组(258.90±54.46ng/L,118.24±36.51ng/L,58.22±2.00ng/Lvs694.36±712.08ng/LP<0.05或0.01),并且随着大黄素浓度的升高,其TGF-β1的含量逐渐降低,但其差异无统计学意义.结论:大黄素可降低PSC中TGF-β1的含量,说明大黄素可以通过抑制TGF-β1的表达发挥防治胰腺纤维化的作用.AIM： To observe effect of emodin on TGF-β1 in rat pancreatic stellate cells. METHODS： We used SD male rats to identify, isolate, and culture pancreatic stellate shaped cells in vitro, and after passage, we used ELASA to observe effect of emodin on TGF-betal in rat pancreatic stellate cells. RESULTS： The TGF-betal content of pancreatic stellate cell of emodin in different concentrations was lower than that of control group （258.90 ±54.46 ng/L, 118.24 ±36.51 ng/L, 58.22 ± 2.00 ng/L vs 694.36± 712.08 ng/L, P 〈 0.05 or 0.01）. As the concentration of emodin increased, TGF-β1 content was reduced accordingly. However, no significant difference was observed. CONCLUSION： Emodin can cut down TGF-β1 content of pancreatic stellate cells, which confirms emodin can prevent and cure pancreatic fibrosis by inhibiting expression of TGF-β1.

关 键 词：胰腺纤维化 大黄素 转化生长因子-Β1 

分 类 号：R285.5[医药卫生—中药学]