微小卡罗藻溶血活性的生长期特征及其溶血毒素种类分析  

Hemolytic activity of Karlodinium micrum at different growth phases and analysis of hemolysin species

作　　者：周成旭[1] 傅永静[1] 陈清峰[1] 严小军[1] 

机构地区：[1]宁波大学,海洋生物工程重点实验室,浙江宁波315211

出　　处：《海洋学报》2009年第2期146-151,共6页

基　　金：科技部中国欧盟科技合作项目(国科外〔2005〕262-0505);教育部长江学者与创新团队项目(IRT0734);宁波大学学科项目(XK0613046);国家科技支撑计划项目(2007BAD43B07)

摘　　要：研究微小卡罗藻的生长曲线及不同生长期藻细胞提取液和去藻上清液溶血活性的大小,通过乙酸乙酯和Bligh-Dyer两种方法提取溶血毒素,利用柱层析和薄层层析对溶血毒素成分进行初步的分离与分析。结果表明,藻细胞提取液在4个生长阶段中都具有明显的溶血活性,去藻上清液在平稳期和衰亡期具有明显的溶血活性,延滞期及对数生长期溶血活性很低,表明微小卡罗藻的溶血物质是其自身正常生理代谢产物,且在细胞老化过程中可以释放至水体环境中。两种提取方法都可以将溶血毒素提取出来,进一步的色谱分离和生物活性检测发现微小卡罗藻主要含有3种不同性质的溶血毒素,其溶血作用可能与微小卡罗藻较高含量的多不饱和脂肪酸18:5n3和22:6n3有关。Hemolytic activity of the cell lysate and culture medium of Karlodiniurn rnicrum were tested at various algal growth phases, as well as to compare the difference between ethyl acetate and Bligh--Dyer, the major components of the hemolysin were fractionated with column chromatography and thin-layer chromatography. The results showed that the hemolytic activity in cells of K. rnicrum at the four growth phases maintained at a high level, whereas high hemolytic activity was only observed in the stationary and senescent phases in the filtrates the culture media, indicating that the hemolysin of K. micrum are normal physiological metabolites, and can be released into the environment when cells are in aging process. Ethyl acetate extraction and Bligh-Dyer extraction are both feasible to extract the hemolysin. Through chromatographic separation and hemolytic activity assay tracking, we found that there were three major hemolysin fractions in important r Key words： K. micrum, and two polyunsaturated fatty acid, namely 18 ： 5n3 and 22 ： 6n3, may play an ole in hemolysis.

关 键 词：微小卡罗藻 溶血毒素 生长周期 

分 类 号：Q179.1[生物学—水生生物学]