玉米PEPC基因在籼型水稻保持系不同遗传背景下的效应及转PEPC基因后代的耐光氧化特性  

Effect of Maize PEPC Gene in Different Genetic Backgrounds of CMS Maintainers and Tolerance to Photooxidation in the PEPC Transgenic Line

作　　者：向珣朝[1,2] 何立斌[2] 孙建明[1] 李季航[2] 姚嫣萍[1] 李平[2] 

机构地区：[1]西南科技大学植物分子遗传育种实验室,四川绵阳621010 [2]四川农业大学水稻研究所,四川温江611130

出　　处：《中国水稻科学》2009年第3期257-262,共6页Chinese Journal of Rice Science

基　　金：四川省科技厅资助项目(2008JY0115-1);国家863计划资助项目(2006AA10Z1D1);四川省教育厅重点项目(07ZA130);西南科技大学博士基金资助项目(07zx0101)

摘　　要：分别以转PEPCKitaake和Kitaake作父本,以4份不同来源的保持系珍汕97B、K17B、Ⅱ-32B和G2480B为母本构建F1,考查PEPC的表达及其对光合性状和农艺性状的影响。导入PEPC后,水稻净光合速率、气孔导度、胞间CO2浓度和PEPC酶活性均表现为增加,最突出的是其PEPC酶活性均成倍增加。蒸腾速率方面,只有K17B/PEPC表现为下降。珍汕97B的净光合速率、气孔导度和PEPC酶活性一般配合力相对效应值均最大。在农艺性状上,G2480B/PEPC的有效穗数、结实率和单株产量均大幅度提高,分别增加了31.46%、1.39倍和1.83倍,千粒重也增加了7.84%。珍汕97B/PEPC由于穗变短,即使结实率和千粒重大幅度增加,减产仍达9.84%。G2480B的穗长、每穗粒数、结实率和单株产量一般配合力相对效应值均最大,而珍汕97B最小。在耐光氧化方面,转PEPC基因Kitaake明显优于Kitaake。说明PEPC单个基因对改善籼型三系保持系的光合性能和增加产量有较重要的作用。在供试的4份保持系中选用保持系宜考虑G2480B,PEPC在此遗传背景下的表达丰度较高,提高结实率和单株产量等农艺性状的正效应最好。To examine the effects of PEPC expression on photosynthetic traits and agronomic traits, eight crosses were made using Kitaake with PEPC and Kitaake as male parents and Zhenshan 97B, K17B, l1-32B and G2480B as female parents, respectively. All the F1 s of combinations with PEPC were increased in the net photosynthetic rate （Pn）, stomatal conductance, intercellular CO2 concentration and PEPCase activity in leaves. There was only a decrease in K17B/PEPC for transpiration rate. Relative values of general combining ability （GCA） of Pn, stomatal conductance, and PEPCase activity in leaves for Zhenshan 97B were larger than those for the other maintainer lines. The number of effective panicles, seed setting rate and grain yield per plant of G2480B/PEPC were increased by 31.46%, 1.39-fold and 1.83-fold, respectively, and the 1000-grain weight was increased by 7.84 %. Even if the seed setting rate and 1000-grain weight of Zhenshan 97B/PEPC increased significantly,the grain yield per plant decreased significantly by 9.84% because of shortened panicle length. Relative values of GCA of panicle length, number of spikelets per panicle, seed setting rate and grain yield per plant for G2480B were the largest among the tested maintainer lines, and the smallest for Zhenshan 97B. Kitaake with PEPC was more tolerant to photooxidation than Kitaake. The results suggest that PEPC has an important effect on increasing photosynthetic capacity and grain yield of the maintainer lines. G2480B should he selected when to screen indica maintainer by molecular marker-assisted selection, because expression abundance of PEPC is higher and its positive effect on increasing seed setting rate and grain yield per plant is the best.

关 键 词：磷酸烯醇式丙酮酸羧化酶基因 细胞质雄性不育 保持系 遗传背景 光氧化 基因效应 转基因 

分 类 号：S511.21[农业科学—作物学]