医用胶原膜浸提液对CHL细胞染色体畸变作用的研究  

Study on the Effects of Medical Collagen Membrane on Chromosome Aberration in CHL Cells

作　　者：吕静 李练兵 马明福 李新生 张其清[2] 

机构地区：[1]重庆市人口和计划生育科学技术研究院,400020 [2]中国医学科学院生物医学工程研究所

出　　处：《中国计划生育学杂志》2009年第5期271-273,共3页Chinese Journal of Family Planning

摘　　要：目的:研究医用胶原膜用于医学领域的遗传毒性。方法:在代谢活化和非代谢活化测试系统下对胶原膜浸提液进行中国仓鼠肺(CHL)细胞的染色体畸变试验。结果:胶原膜浸提液终浓度在31.25～250μl/ml时,观察24h和48h收集的染色体,畸变率均在正常范围(<5%)。直接诱变剂丝裂霉素C(0.20μg/ml),在非代谢活化时染色体畸变率为31%～40%,间接诱变剂环磷酰胺(60μg/ml)在S9代谢活化时染色体畸变率为37%～46%,均使染色体畸变率增加。结论:在本实验条件下医用胶原膜浸提液未诱发CHL细胞染色体畸变率增加。Objective： To investigate the genotoxicity of medical collagen membrane applied in medical field. Methods： The chromosome aberration test of Chinese hamster lung （CHL） cells was conducted in collagen membrane extracts under the metabolie and non - metabolic activation systems. Results： The chromosome samples were collected at 24h and 48h of observation, and the aberration rates were under the normal limits （ 〈 5% ） when the final concentrations of collagen membrane extracts were 31.25-250trl/ml. While the aberration rates under non- metabolic activation increased to 31 -40% when the concentration of direct clastogen mitomycin was 0.20μg/ml, and the aberration rates under S9 metabolic activation were 37 - 46% with 60μg/ml indirect clastogen cyclophosphamide, which showed that the clastogens could induce the significant increase of chromosome aberration rates. Conclusion： The medical collagen membrane extracts do not induce the increase of chromosome aberration rates in CHL ceils under the experiment conditions of this study.

关 键 词：医用生物材料 胶原膜 浸提液 中国仓鼠肺细胞 染色体畸变试验 

分 类 号：R318.08[医药卫生—生物医学工程]