mecA基因在金黄色葡萄球菌中的分布及对耐药性的影响  

Distribution of mecA in Staphylococcus aureus and Its Effect on Antibiotics-resistance

作　　者：陈庆增[1] 罗兵[2] 孙迎娟[1] 黄伟丽[1] 秦萍[1] 

机构地区：[1]青岛大学医学院附属医院院感科,山东青岛266003 [2]青岛大学医学院微生物学教研室,山东青岛266012

出　　处：《中华医院感染学杂志》2009年第9期1028-1031,共4页Chinese Journal of Nosocomiology

摘　　要：目的探讨mecA基因在金黄色葡萄球菌中的分布以及对其耐药性的影响。方法收集金黄色葡萄球菌临床分离菌株47株,用琼脂扩散法进行敏感性检测,提取分离株DNA,通过聚合酶链反应(PCR)对mecA基因进行扩增,分析mecA基因和金黄色葡萄球菌耐药性的关系。结果47株金黄色葡萄球菌中,有33株为耐甲氧西林金黄色葡萄球菌(MRSA),占70.2%;甲氧西林敏感金黄色葡萄球菌(MSSA)有14株,占29.8%;在33株MRSA中,只有3株对除替考拉宁、万古霉素等糖肽类以外的抗菌药物敏感,占9.1%;14株MSSA中只有2株对全部12种抗菌药物敏感,仅占14.3%;PCR结果显示,在33株MRSA中,32株携带mecA基因,占97.0%;而在14株MSSA中有3株携带mecA基因,3株携带mecA基因的MSSA耐药性较不携带mecA基因MSSA的耐药性严重。结论金黄色葡萄球菌中MRSA具有较高的分离率,MRSA的耐药性非常严重,万古霉素等糖肽类抗菌药物是惟一有效的抗菌药物,绝大多数的MRSA携带有mecA基因,mecA基因在金黄色葡萄球菌的耐药机制中发挥重要作用,携带mecA基因的MSSA耐药情况比不携带mecA基因的MSSA严重。OBJECTIVE To understand mecA gene distribution in Staphylococcusaureus and its role in antibiotics- resistance. METHODS In this study, a total of 47 S. aureus strains were isolated from hospitalized patients. Agar disk diffusion test was conducted to determine the resistance of S. aureus to antibiotics. The DNA of these strains were extracted and purified. The mecA gene was tested by PCR and the relation between the mecA gene and antibiotics-resistance was analyzed. RESULTS Of 47 strains, 33（70. 2%） were MRSA. Of 33 MRSA, only 3 strains were susceptible to glycopeptides antibiotics. Only 2 strains （14.3%） of 14 MSSA were susceptible to all of the 12 antibotics. The results of PCR revealed that 32 out of 33 MRSA （97.0%） carried mecA in their genome. One strain was mecA gene negative. Among 14 MSSA, 3 （21.4%）strains carried mecA gene. CONCLUSIONS The isolation rate of MRSA in S. aureus is high. The resistance to antibiotics of MRSA is popular Gtycopeptides antibiotics. Most of MRSA carry mecA gene, which plays an important role in antibiotics-resistance. Fewer MSSA carry mecA gene.

关 键 词：MECA基因 金黄色葡萄球菌 耐甲氧西林金黄色葡萄球菌 耐药性 

分 类 号：R378.11[医药卫生—病原生物学]