OXA-23碳青酶烯酶在鲍氏不动杆菌烧伤分离株中流行  

Carbapenemase of OXA-23 Prevailed in Acinetobacter baumannii Isolated from Burned Patients

作　　者：潘宇红[1] 苏青和[2] 黄璇[1] 程华莉[1] 陈蓉芳[1] 张烽[1] 

机构地区：[1]南通大学附属第三医院检验科 [2]南通大学附属第三医院烧伤科

出　　处：《中华医院感染学杂志》2009年第10期1175-1177,共3页Chinese Journal of Nosocomiology

摘　　要：目的了解烧伤科鲍氏不动杆菌分离株碳青酶烯酶的分布情况。方法测定20株分离自烧伤科鲍氏不动杆菌对20种抗菌药物的敏感性,采用PCR方法进行OXA-23群、OXA-24群、OXA-51群、OXA-58群、IMP、VIM及SIM等7种碳青酶烯酶基因检测,并对部分阳性产物进行基因测序。结果烧伤科20株鲍氏不动杆菌分离株对β-内酰胺类抗菌药物耐药率除左氧氟沙星和头孢哌酮/舒巴坦外均>85%,PCR检测OXA-23群基因19株(95%)呈阳性,其他碳青酶烯酶基因均为阴性;部分阳性产物经测序比对与OXA-23群基因CAB69042.1相同。结论烧伤科鲍氏不动杆菌分离株对β-内酰胺类抗菌药物多药耐药非常严重,OXA-23碳青酶烯酶在鲍氏不动杆菌烧伤分离株中流行。OBJECTIVE To investigate the disseminateion of the carbapenemase in Acinetobacter baumannii isolated from burned patients. METHODS Susceptibility tests of 20 strains of A. baumannii isolated from burned patients to 20 kinds of antimicrobial drugs were detected. Subsequently, seven genes encoding carbapenemase, including OXA-23, OXA-24, OXA-51, OXA-58, IMP, VIM and SIM, were detected by PCR and genes of partial positive products were sequenced. RESULTS Drug resistances to β-lactam antibiotics of 20 strains of A. baumannii isolated from burned patients all exceeded 85 % except to levofloxacin or cefoperazone/sulbactam. Nineteen strains （95%） were OXA-23 genes positive and all other genes were negative. Through sequence and Blast analysis, OXA-23 gene products were identical with that of CAB69042.1. CONCLUSIONS The multidrug resistance of A. baumannii isolated from burned patients to β-lactam antibiotics is critical. Carbapenemase of OXA-23 prevails in A. bauman- nii isolated from burned patients.

关 键 词：碳青酶烯酶 鲍氏不动杆菌 Β-内酰胺类 

分 类 号：R378[医药卫生—病原生物学]