地震患者耐甲氧西林金黄色葡萄球菌基因分型及同源性比较研究  

Genotyping and Homology Comparison of Meticillinresistant Staphylococcus aureus Isolated from Earthquake Victims

作　　者：叶远馨[1] 范红[1] 陆小军[1] 王军[1] 郭靓[1] 丁柳[1] 庄杰[1] 陈慧莉[1] 

机构地区：[1]四川大学华西医院临床微生物及分子诊断实验室,四川成都610041

出　　处：《中华医院感染学杂志》2009年第10期1189-1192,共4页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金(NSFC)资助(30470761);美国纽约中华医学基金会(CMB)"感染性疾病研究"项目资助(06838)

摘　　要：目的了解从地震受伤患者分离到的耐甲氧西林金黄色葡萄球菌(MRSA)基因特征,并与同期非灾区患者分离的MRSA进行比较。方法用葡萄球菌盒式染色体(SCCmec)多重PCR分型技术进行基因分型,并检测其杀白细胞毒素基因(PVL),采用Diversilab进行同源性比较,并进行药敏分析。结果地震受伤患者与非灾区患者分离到的MRSA均表现为SCCmecⅢ型,PVL阴性;同源性分析显示,分离到的MRSA与社区获得性MRSA代表株中MLST分型ST8型同源性相对较好。结论医院获得性MRSA与社区获得性MRSA可能存在交叉传播,在灾后急救现场及当地医院要加强无菌观念,高度警惕医源性交叉感染的传播。OBJECTIVE To investigate the genetic feature of meticillin-resistant Staphylococcus aures isolated from earthquake victims and compare them with those of common patients. METHODS The MRSA isolates underwent SCCmec（Staphylococcal Cassette Chromosome mec） multi-PCR and PVL gene detection. Diversilab method was used for homology comparison and antibiotic resistance was also analyzed. RESULTS All the MRSA isolates were confirmed to be type Ⅲ SCCmec with PVL gene negative. Homology analysis showed relatively consistent with community-acquired MRSA confirmed to be ST8 by MLST typing. CONCLUSIONS There may be cross-spread of MRSA between hospital and community. We should pay more attation even in emergent time.

关 键 词：耐甲氧西林金黄色葡萄球菌 基因分型 同源性 

分 类 号：R378.11[医药卫生—病原生物学]