高效液相色谱法测定同仁牛黄清心片中芍药苷的含量  

Quantitative Determination of Paeoniflorin in TongRen Niuhuang Qingxin Tablet by HPLC

作　　者：李志猛[1] 杜树山[2] 李向日[1,3] 

机构地区：[1]北京同仁堂股份有限公司科学研究所,北京100079 [2]北京师范大学,北京100875 [3]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2009年第5期1-3,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京市科委重大攻关课题(h050230130910)

摘　　要：目的:采用反相高效液相色谱法测定同仁牛黄清心片中芍药苷的含量。方法:以甲醇-0.05mol·L-1磷酸二氢钾(25∶75)为流动相,Symmetry ShieldTM Rp18(3.9mm×150mm,5μm)为固定相,流速为0.8mL·min-1,检测波长为230nm,柱温为30℃。结果:芍药苷在0.1026～0.5130μg范围内呈现良好的线性关系,回收率为98.94%,RSD为2.05%。结论:该方法简单、准确、灵敏度高可用于同仁牛黄清心片中芍药苷的含量检测。Objective： To develop an HPLC method for quantitave determination of paeoniflorin in Tongren Niuhuang Qingxin Tablet. Method： The chromatographic column was Symmetry Shield^TM Rp18 （3.9 mm × 150 mm, 5 μm）, the mobile phase was methanol-0.05 mol·L^-1 potassium dihydrogen phosphate（25：75） with a flow velocity of 0.8 mL·min^-1 . The detection wave-length was 230 nm, and the column temperature was 30 ℃. Results： It was showed that paeoniflorin had a good linear relationship between the concentrations and peak areas in （0.102 6 - 0.513 0）μg, and the average recovery was 98.94% （RSD = 2.05 % ）. Conclusion： This procedure is highly sensitive, and easy to be performed, which can be used in the quantitative determination of paeoniflorin in Tongren Niuhuang Qingxin Tablet.

关 键 词：同仁牛黄清心片 芍药苷 含量 

分 类 号：R284.1[医药卫生—中药学]