大孔吸附树脂分离纯化丹皮总苷工艺研究  

Study on Separation and Purification Total Glycosides of Tree Peony Bark by Macroporous Resin

作　　者：潘君[1] 余庆华[1] 桂双英[1,2] 刘景标[1] 戴敏[1,2] 

机构地区：[1]安徽中医学院药学院,安徽合肥230031 [2]安徽省现代中药工程技术研究中心,安徽合肥230031

出　　处：《中国实验方剂学杂志》2009年第5期21-24,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：科技部十一五支撑计划课题(2006BAI06A18-07)

摘　　要：目的:优选大孔吸附树脂富集纯化牡丹皮中丹皮总苷的最佳工艺参数。方法:采用HPLC法测定丹皮总苷含量,考察D101、D201、D301、D401、AB-8、NKA-96种大孔树脂对芍药苷的吸附和解吸附性能,并进一步考察分离纯化条件。结果:D101大孔树脂对丹皮总苷提取液纯化最优,上样液芍药苷浓度为0.83mg·mL-1、pH6.5、洗脱流速1BV·h-1,分离纯化条件为:先用10BV蒸馏水洗脱,然后用4BV70%乙醇洗脱,收集70%乙醇洗脱液,浓缩、干燥,即得到丹皮总苷。结论:D101大孔树脂可用于牡丹皮水提取液中丹皮总苷的富集纯化。Objective：To optimize the technical parameters of enrichment and purification of the total glycosides in tree peony bark with macroporous resin. Methods： HPLC method was used to analyze the content of total glycosides,the adsorption and desorption performance of D101, D201, D301, D401, AB-8, NKA-9 were investigated, and the purification of conditions were inspected. Results： The best maeroporous resin for purified extractive of total glycosides in tree peony bark was D101, the concentration of Paeoniflorin was 1.83 mg·mL^-1 with pH 6.5, and with the elution flow rate of 1 BV·h^-1 . Purification of the conditions were as follows ： 10 BV water and 4 BV 70 % ethanol were used as eluant of paeoniflorin, respectively. Portions of 70 % ethanol were collected, concentrated, and dried. Conclusion： D101 resin can be used to refine the total glycosides in the water extracts of tree peony bark.

关 键 词：大孔树脂 吸附 纯化 丹皮总苷 

分 类 号：R286.3[医药卫生—中药学]