体外法添加苹果酸与饱和脂肪酸对瘤胃功能菌群数量的影响  

The Effect of Supplementing with Malic Acid and Stearic Acid on Rumen Functional Microbe in vitro

作　　者：李旦[1,2] 王加启[1] 卜登攀[1] 刘亮[1] 刘开朗[1] 赵圣国[1,2] 于萍[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100193 [2]甘肃农业大学,甘肃兰州730070

出　　处：《微生物学通报》2009年第5期694-699,共6页Microbiology China

基　　金：国家“十一五”科技攻关奶业重大专项(No.2006BAD04A003,2006BAD12B08);动物营养学国家重点实验室自主研究课题[No.2004DA125184(团)0801]

摘　　要：本试验在添加饱和脂肪酸即硬脂酸(Stearic acid,SA)的基础上添加不同水平的苹果酸(Malic acid,MA),采用体外法对瘤胃微生物进行培养,通过实时定量(Real-time)PCR方法检测在添加脂肪酸和苹果酸后,对瘤胃功能微生物如纤毛虫、产甲烷菌,纤维分解细菌、氢化细菌以及脂肪分解菌的数量的影响。实验结果表明,添加硬脂酸后对脂解厌氧弧杆菌(Anaerovibrio lipolytica)和产琥珀酸丝状杆菌(Fibrobacter succinogenes)数量产生了显著影响,脂解厌氧弧杆菌数量减少了95.8%(P<0.001),产琥珀酸丝状杆菌数量增加了52.5%(P<0.05)。添加苹果酸后,添加5mmol/L MA处理组的脂解厌氧弧杆菌数量减少了91.2%(P<0.001),添加10mmol/L MA处理组,减少了94.8%(P<0.001),而MA10组比MA5组的该菌数量减少了41.3%(P<0.05)。本研究结论是硬脂酸和苹果酸分别对瘤胃部分微生物产生了显著的影响。二者对瘤胃微生物的互作影响并不显著。Using real-time PCR to detect the effect of supplementing with Malic acid and stearic acid on rumen main functional microbe, including ciliate protozoa, methanogen, cellulolytic bacteria, hydrogenated bacteria and lipolysis bacteria in vitro. The result showed there were significant effect on the quantities of Anaerovibrio lipolytica, which decreased 95.8%（P〈0.001）, and Fibrobacter succinogenes, which increased 52.5%（P〈0.05） after supplemented with Stearic Acid. The quantities of Anaerovibrio lipolytica were decreased by 91.2%（P〈0.001） on 5 mmol/L MA group and 94.8%（P〈0.001） on 10 mmol/L MA group, and the latter were decreased 41.3%（P〈0.05） than the former. The conclusion was stearic acid and malic acid were significantly effected on part of rumen microbe respectively, however, both of them were not obviously effected on them.

关 键 词：硬脂酸 苹果酸 Real—time PCR 

分 类 号：S852.6[农业科学—基础兽医学]