HIV相关性口腔念珠菌RAPD多态性分析  

Analysis on RAPD Genetic Polymorphism of HIV Related Oral Candida Species

作　　者：武有聪[1] 白丽[1] 袁有华[1] 郭利军[1] 

机构地区：[1]大理学院基础医学院医学微生物学及免疫学教研室,云南大理671000

出　　处：《微生物学通报》2009年第5期728-734,共7页Microbiology China

基　　金：国家自然科学基金项目(No.C30260005)

摘　　要：运用RAPD技术对60株分离自HIV感染者口腔的念珠菌进行分析,其中P2随机引物扩增条带数量0～5条,大小300bp～2kb,白色念珠菌以300bp、400bp和600bp3个主条带为主,非白色念珠菌也存在类似条带。采用SPSS13.0聚类分析,分为5个基因群,14个基因型,白色念珠菌主要为B群。其中P385和P403对5-氟孢嘧啶耐药,聚为C1基因型(欧氏距离平方为0.115),P321和P522对两性霉素B耐药,聚为D1基因型(欧氏距离平方为0.221)。表明HIV感染者口腔来源的念珠菌存在丰富的基因多态性,RAPD技术对于白色念珠菌的基因型鉴定具有重要意义;念珠菌不同种间具有相对特征性的条带,同种不同株间存在相似的条带特征;某些条带特征可能与念珠菌的耐药性相关。60 Candida strains were analyzed with randomly amplified polymorphic DNA（RAPD） from HIV-infected patients＇ oral cavity. The results showed that RAPD-PCR fingerprinting appeared from zero to five bands whose size rang from 300 bp to 2 kb with P2 primer, Candida albicans have three characteristic bands of 300 bp, 400 bp and 600 bp in size, non-Candida albicans have the similar bands. They can be classified five genetic clusters and 14 genotypes by Cluster Analysis, two Candida albicans（P385＆P403） with fluorocytosine-resistance were classified into C1 genotype for ＂Squared Euclidean Distance＂ being 0.115, one Candida albicans（P321） and one Candida glabrata（P522） who were resistant against Amphotericin B were classified into D1 genotype for ＂Squared Euclidean Distance＂ being 0.221. So, an abundant genetic polymorphism appeared in Candida species related HIV, RAPD could be as a reference for genotype of Candida albicans; different Candida speices had some special bands, different Candida strains had similar bands in the same species; it is perhaps that some genotypes were relevant to drug resistance.

关 键 词：基因多态性 药物敏感性 念珠菌 人类免疫缺陷病毒 

分 类 号：R512.91[医药卫生—内科学] R379[医药卫生—临床医学]