HPLC法测定注射用炎琥宁中脱水穿心莲内酯琥珀酸半酯的含量  

Determination of dehydroandrographolide succinate in potassium sodium pehydroandrographolide succinate for injection by HPLC

作　　者：黄瑾 

机构地区：[1]福州市药品检验所,福州350007

出　　处：《海峡药学》2009年第4期53-54,共2页Strait Pharmaceutical Journal

摘　　要：目的建立HPLC法测定注射用炎琥宁中脱水穿心莲内酯琥珀酸半酯的含量。方法色谱柱watersC18(4.6μm×150μm),乙腈-0.05mol.mL-1的磷酸二氢钾溶液(35∶65)为流动相,流速为1.0mL.min-1,检测波长251nm。结果实验结果表明,脱水穿心莲内酯琥珀酸半酯在39.87μg.mL-1-398.70μg.mL-1范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率为100.34%,RSD=0.25%(n=9)。结论该方法简便快速,重现性好,可用于炎琥宁的质量控制。OBJECTIVE To establish a HPLC method for detemination of dehydroandrographolide succinate in potassium sodium pehydroandrographolide succinate for injection. METHODS The waters Ci8（4.6μm× 150μm） was used, the mobile phase consisted of acetoniteile-0.05mol· mL- 1 potassiun dihydrogen phosphate（35 ： 65） with flow rate of 1.0mL· min-1. The detection wavelength was 251nm. RESULTS The method was linear with in the range of 39.87μg· mL- 1 - 398.70μg· mL- 1 （r = 0. 9999） . The average recovery was 100.34 %, and RSD was 0.25 % （n = 9）. CONCLUSION The method is efficient and accurate with a good repeatability and can be using for the control of potassium sodium pehydroandrographolide succinate for injection.

关 键 词：HPLC法 注射用炎琥宁 脱水穿心莲内酯琥珀酸半酯 

分 类 号：R927.3[医药卫生—药学]