应用PCR/RFLP对我国北方斑点热群立克次体分离株的基因型分析  

Genotypic Analysis of Spotted Fever Group Rickettsia Isolated in the North Part of China by PCR/RFLP

作　　者：吴益民[1] 刘昕昕[1] 魏安明[1] 胡玲美[1] 杨青[1] 

机构地区：[1]沈阳军区军事医学研究所

出　　处：《中国公共卫生学报》1998年第2期71-72,共2页

摘　　要：应用立克次体１９０ＫＤ蛋白基因的ＰＣＲ／ＲＦＬＰ分析我国北方斑点热群立克次体分离株的基因型，并和国际标准株作了比较。结果，分离自黑龙江省、内蒙、新疆等地不同蜱类的５个，分离株呈现２种不同的ＲＦＬＰ图谱：ＪＨ－７４株、Ｈｕ－８４株及ＨＬ－８４株和西伯利亚立克次体相同；黑龙江立克次体５４株、３６株二者相同，与其它相关菌株均有差异。基因型结构分析证明，我国北方斑点热群立克次体分离株存在２个种，即西伯利亚立克次体种和斑点热群新种—黑龙江立克次体。Restrictionendonuclease fragment length polymorphism analysis (RFLP)of polymerase chain reaction (PCR) amplified DNA was used to characterize the genotypic diversity of Spotted Fever Group SFG) Rickettsiosis isolated in the North of China.The 5 isolated strains of SFG Rickettsiosis were divided into two genotypes by the Rsa Ⅰ and Pst Ⅰ endonucleases digestion of DNA derived from the R.rickettsii 190 KD antigen gene primers.The JH74 Hu84 strain.H184 strain and R.sibirica 246 strain have identical genotype.The R.heilorgjiangi 54 strain and 36 strain and identical on genotype level and different from the others.Genotypic analysis suggested that the SFGR in the North of Chins existed 2 speices at least,the R.sibirica species and the R.heilongjiangi species.

关 键 词：斑点热 基因型分析 聚合酶链反应 SFGR PDR/RFLP 

分 类 号：R513[医药卫生—内科学] R376[医药卫生—临床医学]