热应激大鼠肝线粒体微粒体脂质过氧化与钙镁三磷酸腺苷酶的变化  

Changes of Ca-Mg-ATPase and Lipid Peroxidation in Liver Mitochondria Microsomes of Heatstressed Rats

作　　者：汤平涛[1] 王瑞波[1] 吴广林[1] 张裕曾[1] 贺涵贞[1] 张国高[1] 

机构地区：[1]同济医科大学职业医学研究所 [2]深圳市卫生防疫站

出　　处：《中国公共卫生学报》1998年第2期76-68,共1页

基　　金：国家自然科学基金;湖北省科学基金

摘　　要：为探讨高温对机体的损伤机理和防治措施，对热应激过程肝线粒体、微粒体脂质过氧化、钙镁三磷酸腺苷酶（Ｃａ－Ｍｇ－ＡＴＰａｓｅ）的变化与联系进行了观察。结果显示，受热大鼠肝线粒体、微粒体丙二醛（ＭＤＡ）含量明显增多，并随受热时间延长而升高；受热（４２℃）１２０ｍｉｎ后大量的肝细胞变性、坏死，超微结构改变明显。脱离热接触６ｈ，肝线粒体ＭＤＡ含量恢复到对照组水平，但微粒体ＭＤＡ明显升高。肝线粒体ＭＤＡ含量增多，Ｃａ－Ｍｇ－ＡＴＰａｓｅ活性下降；ＭＤＡ含量降至对照组水平时，Ｃａ－Ｍｇ－ＡＴＰａｓｅ活性恢复到对照组水平。提示，经一定时间热应激后脱离热环境后，肝线粒体脂质过氧化可恢复，而微粒体脂质过氧化不易恢复；热应激肝线粒体ＭＤＡ含量与Ｃａ－Ｍｇ－ＡＴＰａｓｅ活性变化存在一定联系。The liver mitochodria,microsomal Ca -Mg-ATPase,superoxide dismutase (SOD),malondialdehyde (MDA),mitochondrial glutathione peroxidase(GSHPx),cytosolic Ca  were detected;and the Ultrastructural changes of hepatocyte were observed during heatstressed.The mitochondria,microsomal MDA were increased significatly (P<005) in 60 min and 120 min groups,and were increased by prolonged heat time.The ultrastructural changes,degeneration and necroses were occurred in a number of liver cells at heated 120 min.At recovery 6 h (after heatstressed 60 min),mitochondrial MDA recoveried to level of control group,but microsomal MDA increased significantly (P<0 05).The mitochondrial MDA increased,CaMg ATPase decreased,and MDA decreased,Ca Mg ATPase recoveried at the same time.The mitochondrial GSHPx,SOD showed up tendency to increase,MDA to decrease in microsomes mitochondria of the heatstressed rats following Setreated.The paper suggested that mitochodrial lipid peroxidation may be recoveried to normal after heatstressed in short time,however microsomal lipid peroxidaton not easy.There is the interrelation between MDA and Ca Mg ATPase of mitochodria in the heat tress condition.The ffect of Se on antilipid peroxidation in heat stress was going to be reseached.

关 键 词：热应激 线粒体 微粒体 脂质过氧化 

分 类 号：R594.1[医药卫生—内科学]