电针“足三里”“三阴交”穴对胶原性关节炎大鼠滑膜细胞分泌功能的影响  

Effect of Electroacupuncture at "Zusanli" (ST 36) and "Sanyinjiao" (SP 6) on Collagen-induced Arthritis and Secretory Function of Knee-joint Synoviocytes in Rats

作　　者：方剑乔[1] 邵晓梅[1] 马桂芝[1] 

机构地区：[1]浙江中医药大学第三临床医学院,杭州310053

出　　处：《针刺研究》2009年第2期93-96,共4页Acupuncture Research

基　　金：国家自然科学基金资助项目(No.30371802);国家重点基础研究发展计划(2005CB523306)

摘　　要：目的:观察电针"足三里""三阴交"对胶原性关节炎(collagen-induced arthritis,CIA)大鼠的治疗作用,并探讨该疗法调节CIA大鼠滑膜细胞分泌功能的部分机制。方法:36只Wistar大鼠随机分为正常对照组、模型对照组和电针治疗组。模型对照组和电针治疗组采用牛Ⅱ型胶原在大鼠背部正中线作多点皮内注射诱发CIA模型。电针治疗组选取"足三里""三阴交"穴进行电针刺激,频率2Hz,强度2mA,时间30min,连续治疗30d。处理前后分别观察CIA大鼠足跖肿胀度、痛阈;采用ELISA法检测大鼠膝关节滑膜细胞培养上清液中前列腺素E2(PGE2)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)水平。结果:模型对照组大鼠足跖肿胀度,滑膜细胞培养上清中PGE2浓度和TNF-α、IL-1β含量均高于正常对照组大鼠,差异有统计学意义(P<0.05,P<0.01),痛阈低于正常对照组大鼠,差异有统计学意义(P<0.05)。与模型对照组比较,电针治疗组大鼠足跖肿胀度减小,痛阈升高,关节滑膜细胞培养上清中PGE2浓度下降,TNF-α和IL-1β含量降低,差异有统计学意义(P<0.05)。结论:电针"足三里""三阴交"穴对胶原性关节炎大鼠具有良好的治疗作用,该作用可能与抑制关节滑膜细胞的分泌功能有关。Objective To observe the effect of electroacupuncture（EA）of ＂Zusanli＂（ST 36）and ＂Sanyinjiao＂（SP 6）on collagen-induced arthritis（CIA）and its underlying mechanism in regulating the secretory function of knee-joint synovial cells in CIA rats.Methods Thirty-six Wistar rats were equally randomized into control,model and EA groups.CIA model（rheumatoid arthritis）was duplicated by intradermal injection of Bovine type Ⅱ collagen into the back of the anesthetized rats.EA（2 Hz,2 mA）was applied to ＂Zusanli＂（ST 36）and ＂Sanyinjiao＂（SP 6）for 30 min,once a day for 30 days.The paw volume was measured by using a Plethysmometer and hot water tail-flick tests（50 ℃）were conducted for detecting the rats＇ pain threshold（PT）before and after the treatment.The contents of PGE2,IL-1β and TNF-α in supernatant of the cultured joint synoviocytes were detected by enzyme linked immunosorbent assay（ELISA）.Results In comparison with control group,the swelled paw volume,the declined PT,PGE2,TNF-α and IL-1β contents in knee-joint synoviocyte supernatant were significantly higher in model group（P〈0.05）.Compared with model group,PT increased obviously（P〈0.05）;paw volume,supernatant PGE2,TNF-α and IL-1β contents decreased obviously（P〈0.05）in EA group.Conclusion EA of ST 36 and SP 6 has a pronounced therapeutic effect in relieving knee joint pain and inflammation in CIA rats,which may be related to its effect in regulating the secretory function of the knee-joint synoviocytes.

关 键 词：胶原性关节炎 电针 膝关节滑膜细胞 肿瘤坏死因子-α 白介素-1β 

分 类 号：R245.97[医药卫生—针灸推拿学]