电针诱导神经生长因子透血脑屏障效应及其机制分析  

Effect of Electroacupuncture on the Permeability of Blood-Brain Barrier for Nerve Growth Factor and Its Relevance to PKC Pathway in Cerebral Ischemia Rats

作　　者：林咸明[1] 谭克平[1] 张爱军[1] 何丽华[1] 

机构地区：[1]浙江中医药大学第三临床医学院针灸教研室,杭州310053

出　　处：《针刺研究》2009年第2期110-113,共4页Acupuncture Research

基　　金：国家中医药管理局资助项目(02-03JL03);浙江省中医药管理局资助项目(2007CB160);浙江省"重中之重"学科(针灸推拿学科)基金资助项目

摘　　要：目的:观察电针"百会""哑门"诱导外源性神经生长因子(NGF)透过血脑屏障的效应及其促透效应与蛋白激酶C(PKC)信号传导通路的相关性。方法:采用急性反复脑缺血再灌注法制造脑缺血学习记忆障碍大鼠模型,3周后,将动物随机分为模型对照组、NGF组(尾静脉注射NGF)、电针组(电针"百会""哑门")、NGF+电针组(尾静脉注射NGF,同时"百会""哑门"通以电针)、NGF+H7+电针组(尾静脉注射NGF后注射异喹啉磺酰类H7,同时"百会""哑门"通以电针),另设假手术组,每组均8只大鼠。采用ELISA法检测各组大鼠脑组织中NGF含量。结果:NGF组大鼠脑组织中NGF含量与模型对照组比较,差异无统计学意义(P>0.05);NGF+电针组大鼠脑组织中NGF含量与假手术组、模型对照组、NGF组及电针组比较均明显升高,差异均有统计学意义(P<0.01);NGF+H7+电针组与NGF+电针组比较,脑组织中NGF含量有下降趋势,但差异不具统计学意义(P>0.05)。结论:"百会""哑门"电针能诱导NGF透过血脑屏障,使大鼠脑组织中NGF含量明显提高,该促透作用与PKC信号通路相关性不明显。Objective To observe the effect of electroacupuncture（EA）of ＂Baihui＂（GV 20）and ＂Yamen＂（GV 15）on the permeability of blood-brain barrier for exogenous nerve growth factor（NGF）and its relevance to PKC pathway in cerebral ischemia（CI）rats.Methods Forty-eight SD rats were randomized into sham-operation（sham）,model,NGF,EA,NGF＋EA,and NGF＋H7＋EA groups.CI model was established by repeated occlusion of the bilateral carotid arteries and reperfusion.NGF（10 μg/kg）was administrated by intravenous injection（vena caudalis）;while H 7 [1-（5-isoquinolinesulfonyl）-2-mehtylpoperazine,an inhibitor of protein kinase C（PKC）,1 mg/kg] was given by intraperitoneal injection.EA（100 Hz,2 mA）was applied to ＂Baihui＂（GV 20）and ＂Yamen＂（GV 15）for 20 min.NGF content in the brain tissue was assayed by enzyme-labeled immunosorbent assay（ELISA）.Results In comparison with sham group,cerebral NGF content had no apparent change in model group（P〉0.05）.Comparison among the abovementioned 6 groups showed that cerebral NGF level in NGF＋EA group was significantly higher than those in sham,model,EA and NGF groups（P〈0.01）.No significant differences were found among sham,model,NGF,EA and NGF＋H7＋EA groups and between NGF＋EA and NGF＋H7＋EA groups in cerebral NGF contents（P〉0.05）.Conclusion EA of GV 20 and GV 15 can raise the permeability of blood-brain barrier for NGF to increase its level in the cerebral tissue in CI rats,favoring the regeneration and repair of nerves,which has no clear relevance to PKC pathway.

关 键 词：脑缺血 电针 神经生长因子 血脑屏障 蛋白激酶C通路 

分 类 号：R245.97[医药卫生—针灸推拿学]