阳春砂仁中总黄酮、异槲皮苷和槲皮苷含量测定研究  

Determination of the Contents of Total Flavonoids,Isoquercitroside and Quercitroside in Amomum Villosum

作　　者：李宗主[1] 潘瑞乐[1] 李展[1] 赵晓宏[1] 斯建勇[1] 

机构地区：[1]中国医学科学院,北京协和医学院药用植物研究所,北京100193

出　　处：《科技导报》2009年第9期30-33,共4页Science & Technology Review

摘　　要：建立阳春砂仁中异槲皮苷和槲皮苷含量的高效液相(HPLC)测定方法。异槲皮苷进样量在0.0183～0.0915μg范围内有良好的线性关系,平均回收率为102.69%,相对标准偏差为0.70%(n=6);槲皮苷进样量在0.0294～0.1470μg范围内线性关系良好,平均回收率为103.15%,相对标准偏差为0.68%(n=6)。建立了紫外分光光度法测定砂仁中总黄酮含量的方法,采用槲皮苷为对照品,浓度在0～9.8μg/mL范围内线性关系良好,平均回收率为100.89%,相对标准偏差为1.19%(n=6)。本方法简便快捷,可作为砂仁含量测定质量控制方法。This paper studies the determination of the contents of isoquercitroside and quercitroside in Amomum villosum by using HPLC. A good linear relationship exists between the peak areas and the sample contents within the ranges of 0.018 3-0.091 5 μg and 0.029 4-0.147 μg for isoquercitroside and quercitroside, respectively. The average recovery rates of isoquercitroside and quercitroside are 102.69% （n=6, RSD=0.70%） and 103.15% （n=6, RSD=0.68%）, respectively. The UV spectrophotometry is used for determining total flavonoids in Amomum villosum, with quercitroside as the reference, which shows a linear relationship within the range 0-9.8 μg/mL. The average recovery rates of quercitroside are 100.89% （n =6, RSD=1.19%）. The methods are simple, rapid and accurate and can be used for quality control of Amomum villosum.

关 键 词：阳春砂仁 高效液相色谱法 紫外分光光度法 槲皮苷 异槲皮苷 总黄酮 含量测定 

分 类 号：R284[医药卫生—中药学]