聚乳酸聚乙醇酸/RNA Ⅲ抑制肽缓释微球的制备及评价  

Preparation and evaluation of poly(D,L-lactide)-co-gly-collide/RNA Ⅲ inhibiting peptide microspheres controlled delivery systems

作　　者：郝立波[1] 张小斌[1] 王继芳[1] 王岩[1] 邢庆昌[1] 

机构地区：[1]中国人民解放军总医院骨科,北京100853

出　　处：《中华关节外科杂志（电子版）》2009年第2期54-57,共4页Chinese Journal of Joint Surgery(Electronic Edition)

基　　金：国家自然科学基金资助项目(30640088)

摘　　要：目的探讨聚乳酸聚乙醇酸(PLGA)/RNA Ⅲ抑制肽(RIP)缓释微球的合理制备方法,测定其载药率和体外释药特征。方法采用改良复乳-溶剂挥发法制备PLGA/RIP缓释微球,观察其表征,测定缓释微球的载药率和包封率。采用高效液相色谱法测定不同时点PLGA/RIP的释放速度,观察PLGA/RIP微球的体外释药特点。结果采用改良复乳-溶剂挥发法制备的PLGA/RIP缓释微球粒径均匀、表面光滑,包封率约为(68.22±6.20)%,载药率为(15.35±3.26)%。PLGA/RIP释药动力学方程为y=31.016x+59.611,符合Huguchi方程。结论采用改良复乳-溶剂挥发法制备的PLGA/RIP缓释微球粒径均匀、表面光滑,包封率和载药率较高,体外释放动力学符合Huguchi方程,是理想的缓释载药体系。Objective To study the feasibility and preparation method of poly （D, L-lactide）- co-gly-collide/RNA Ⅲ inhibiting peptide microspheres controlled delivery systems, and observe drug-loading and drug-releasing function. Methods Liquid-phase muhiple emulsion method was used to synthesize PLGA/RIP microspheres with the diameter of 50 - 70 μm. The envelopment ratio and drug-carried ratio were measured. The accumulate release and density of RIP in the different time were detected by high performance liquid chromatography, the characteristic of drug release of PLGA/RIP in vitro was also observed. Results The envelopment ratio was （68.22±6. 20 ） % and drug-carried ratio was （ 15.35 ± 3.26）%. The kinetic equation of release of PLGA/RIP is y = 31. 016x ＋ 59. 611. They were coincident with Huguchi equations. Conclusions The PLGA/RIP microspheres＇ envelopment ratio and drug-carried ratio were satisfying. The kinetic equation of release of PLGA/RIP was coincident with Huguchi equations. The PLGA/RIP microsphere is an optimal delayed release drug-loading system.

关 键 词：聚乙醇酸 RNA Ⅲ抑制肽 缓释 

分 类 号：R687.4[医药卫生—骨科学] R943[医药卫生—外科学]