血小板源生长因子诱导血管平滑肌细胞迁移及机构  

Mechanism involved in the Migration ofVascular Smooth Muscle Cells inducedby Platelet-derived Growth Factor

作　　者：谢良地[1,2,3,4] 吴可贵[1,2,3,4] 陈达光[1,2,3,4] A.D.Hughes Z.Clunn J.Lymn 

机构地区：[1]福建医科大学附属第一医院心内科,福建省高血压研究所 [2]福建省教委 [3]英国文化委员会 [4]英国帝国理工医学院临床药理系

出　　处：《中国动脉硬化杂志》1998年第1期10-14,共5页Chinese Journal of Arteriosclerosis

摘　　要：为探讨血小板源生长因子诱导血管平滑肌细胞迁移和细胞内信号传递机制。以体外培养的平滑肌细胞为对象，用血小板源生长因子ＢＢ二聚体作诱导刺激物，采用改良的Ｂｏｙｄｅｎ微孔膜双槽法进行细胞迁移实验，荧光染料Ｆｕｒａ－２法测定细胞内游离钙离子浓度。结果发现，血小板源生长因子ＢＢ二聚体能明显诱导体外培养的平滑肌细胞迁移，使细胞内游离钙离子浓度（［Ｃａ２＋］ｉ）明显升高（Ｐ＜０．０１），峰值浓度位于５ｇ／Ｌ。１００ｍｏｌ／Ｌ钙离子螯合剂ＢＡＰＴＡ和酪氨酸激酶抑制剂５０ｍｏｌ／Ｌ　Ｇｅｎｉｓｔｅｉｎ既能抑制血小板源生长因子－ＢＢ诱发的升高，也能抑制血小板源生长因子对平滑肌细胞的诱导趋化迁移作用。实验结果提示，血小板源生长因子是诱导平滑肌细胞迁移的趋化物；升高是平滑肌细胞对血小板源生长因子反应的早期细胞内信号传递通路之一；血小板源生长因子诱发的平滑肌细胞迁移既依赖于升高，又依赖于酪氨酸蛋白激酶活性。ABSTRACTAim To investigate the intracellular signalling relatedto the migration promoted by platelet-derived growthfactor (PDGF) .Methods Cultured smooth muscle cells(SMC) derivedfrom rats (A7r5) were used. Modified Byoden cham-ber technique was employed for migration accessment.Intracellular free calcium ion concentration was measured using Fure-2. Results PDGF-BB promoted migration of SMC andcaused an increase in of SMC, with peak re-sponse at concentration of PDGF 5 g/L. The migra-tion and increase in induced by PDGF weremarkedly inhibited by 1 00 mol/L BAPTA 1 97 ±30 vs 1 7．7± 6 nmol/L , P < 0.01 ) , a calciumchelator, and 50 mol/L genestein, a tyrosine kinaseinhibitor.Conclusion The increase in plasmic ionic calcium isone of early intracellular signalling after interaction ofPDGF and SMC. The migration of SMC induced byPDGF is either calcium dependent or tyrosine kinasedependent. KEY WORDS Platelet-derived growth factor;Smooth muscle cell; Migration; Intracellular freecalcium

关 键 词：PDGF 平滑肌细胞 迁移 动脉粥样硬化 

分 类 号：R543.502[医药卫生—心血管疾病]