白念珠菌烯醇化酶单克隆抗体的制备及鉴定  

Preparation and identification of monoclonal antibody against enolase of candida albicans

作　　者：宋秋荷[1] 李忠元[2] 赵政凤[3] 王鲁[4,5] 郝飞[4,6] 

机构地区：[1]九江学院临床医学院皮肤科,江西九江332000 [2]九江学院临床医学院急诊科,江西九江332000 [3]九江学院基础医学院,江西九江332000 [4]九江学院 [5]重庆市第一人民医院皮肤科,重庆400010 [6]第三军医大学西南医院皮肤科,重庆400038

出　　处：《西南国防医药》2009年第5期474-477,共4页Medical Journal of National Defending Forces in Southwest China

基　　金：江西省九江市科技局立项课题(200564)

摘　　要：目的:制备并鉴定抗白念珠菌烯醇化酶单克隆抗体杂交瘤细胞株。方法:提取白念珠菌标准株ATCC-9002胞内烯醇化酶做抗原,再用细胞融合技术制备单克隆抗体,采用间接ELISA法对单克隆抗体进行筛选和鉴定。结果:建立了1株持续分泌抗白念珠菌烯醇化酶单克隆抗体的杂交瘤细胞株,其中Mab07.4G6-F3-C7小鼠腹水单克隆抗体的最高稀释度达1:12800,单克隆抗体的重链属lgGl亚类,轻链属κ型;Western blot证实单抗能与白念珠菌烯醇化酶抗原特异性地结合。结论:本研究建立了抗白念珠菌烯醇化酶杂交瘤细胞株单克隆抗体,为快速诊断白念珠菌感染奠定了实验基础。Objective： To prepare and identify monoclonal antibodies （McAbs） against enolase of eandida albicans. Methods ： The enolase from standard line ATCC - 9002 cells of candida albicans was extracted as the antigen. Monoclonal antibodies against enolase of candida albicans were prepared by the lymphocyte hybridoma technique and screened by ELISA. Results ： One cell line of hybridoma constantly secreting McAbs against enolase of candida albicans was constructed successfully. The highest dilute strength of McAbs from Mab07.4G6 - F3 - C7 mouse＇s hydroperitonia reached up to 1：12 800. In addition,the H - chain of McAbs belonged to IgG1 subtype and its L - chain belonged to Kappa type. Western blot showed that the McAbs could specifically link with the antigen of enolase of candida albicans. Conclusion ： The McAbs established by this method is highly specific for enolase of candida albican and it provides a basic condition for development of rapid diagnosis of systemic infection.

关 键 词：白念珠菌 烯醇化酶 杂交瘤 单克隆抗体 

分 类 号：R379.4[医药卫生—病原生物学]