中药通心络超微粉对糖尿病大鼠心肌细胞凋亡的干预作用  

Effect of Tongxinluo Super on Myocardial Apotosis:Experiment with Diabetic Rats

作　　者：刁学织[1] 王小梅[2] 

机构地区：[1]辽宁医学院附属第一医院心内科,辽宁锦州121001 [2]辽宁医学院附属第一医院高级医疗服务中心,辽宁锦州121001

出　　处：《中国误诊学杂志》2009年第16期3789-3790,共2页Chinese Journal of Misdiagnostics

基　　金：辽宁省教育厅高等学校科学研究项目(编号:05L126)

摘　　要：目的:探讨通心络超微粉对糖尿病大鼠心肌细胞凋亡的干预作用,为临床用药提供实验依据。方法:取SD大鼠随机分为3组:正常对照组、糖尿病心肌病变组(Diabetic Myocardi-opathy,DCM组)和通心络干预组(TXL组)。采用Tunel法检测光镜下观察心肌细胞凋亡指数,免疫组化法测定Bcl-2,Bax及P53基因蛋白表达。结果:TXL组大鼠较DCM组大鼠心肌细胞凋亡指数明显下降(P<0.01),Bcl-2表达明显增多(P<0.01),Bax及P53表达明显减少(P<0.01)。结论:通心络超微粉可以通过多种途径有效减少糖尿病大鼠心肌细胞凋亡。Obejective：To study the effect of TXL super on myocardial apotosisof diabetic rats and establish the experimental foundation for clinical application. Methods ：SD rats were randomly divided into 3 groups ：Diabetic myocardiopathy group,TXL group and normal control group. At the end of the experiment,Pieces of myocardium tissue were taken out to undergo apotosis with TUNEL method and related gene expression （Bax, Bcl-2, P53） with immunohistochemical technique by microscopy. Result ：Compared with DCM group,the apotosis index and the expression of Bax, P53 dereased significantly（P〈0. 01） in the TXL group ;and the expression of Bcl-2 increased significantly（P〈0. 01） in the TXL group. Conclusion ：TXL super can markedly prevent myocardial apotosis of diabetic rats from damage through many paths.

关 键 词：心血管药物(中药)/药理学 糖尿病/中药疗法 肌细胞 心脏/药物作用 细胞凋亡/药物作用 大鼠 

分 类 号：R587.1[医药卫生—内分泌]