三磷酸腺苷氯化镁对兔缺血后肝组织保护作用的研究  

The Study of Protecting Effect of ATPMgCl2 Infusion on Ischmic Liver in Rabbits

作　　者：欧琨[1,2] 崔自介[1,2] 

机构地区：[1]山东省滨州医学院附属医院外科 [2]青岛医学院附属医院外科

出　　处：《中国普通外科杂志》1998年第2期73-76,共4页China Journal of General Surgery

摘　　要：家兔全肝缺血３０分钟后，分别给予生理盐水、三磷酸腺苷（ＡＴＰ）和三磷酸腺苷氯化镁（ＡＴＰ－ＭｇＣｌ２），术后第１，３，５天取血测定谷丙转氨酶（ＡＬＴ）、乳酸脱氢酶（ＬＤＨ）和碱性磷酸酶（ＡＬＰ）。术后第１天ＡＴＰ－ＭｇＣｌ２组ＡＬＴ明显低于对照组（Ｐ＜０．０５），而ＡＴＰ组与对照组相比无显著差别；ＡＴＰ－ＭｇＣｌ２及ＡＴＰ组的ＬＤＨ和ＡＬＰ与对照组相比无显著差别。术后２４小时超微结构示：对照组肝明显水肿、细胞器严重损伤和血窦内微小血栓；而ＡＴＰ－ＭｇＣｌ２组细胞轻度水肿，细胞器基本正常，血窦内无微小血栓。实验结果说明ＡＴＰ－ＭｇＣｌ２对缺血肝组织确有保护作用。To evaluate the protective effect of ATP and ATPMgCl2 on hepatic ischemia (HI), we studied HI for 30 minutes in 30 rabbits which were divided into 3 groups. They received 15ml of saline (Control group), ATP, or ATPMgCl2 respectively. On the day 1,3,5 after operation, blood alanine transaminase (ALT), lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) were measured. ALT in ATPMgCl2 group on the first day was significantly lower than that in the control group. But no significant difference in ALT between ATP group and saline group. No significant difference was found among ATP, ATPMgCl2 and control group in LDH and ALP. Ultrastructure showed that 24 hours after HI substantial swollen of hepatic cells, severely damage of organellae and microthrombi in the sinusoid were found in control group, but cells were only slightly swollen, near normal organellae and no thrombi in the sinusiod were found in ATPMgCl2 group. The results suggest that infused ATPMgCl2 has a protective effect on the liver from ischemic injury, and the mechnism of the protection might be due to the improvement of the microciculation and cell swelling.

关 键 词：肝缺血 肝功能 超微结构 ATP-MGCL2 肝组织 

分 类 号：R975.5[医药卫生—药品] R965.2[医药卫生—药学]