人锌指蛋白ZNF256基因克隆、表达谱分析及其功能研究  

Cloning,Expression Profile and Function Analysis of Human Zinc Finger Gene ZNF256

作　　者：任道龙[1] 胡兴旺[1] 徐甜[1] 肖湘文[1] 贺爱兰[1] 颜峰[1] 张健[1] 刘如石[1] 

机构地区：[1]湖南师范大学生命科学学院蛋白质化学与发育生物学教育部重点实验室

出　　处：《激光生物学报》2009年第2期143-147,共5页Acta Laser Biology Sinica

基　　金：湖南省自然科学基金项目(08JJ3082);国家“973”计划项目(2006CB943506);湖南省大型精密仪器运行及实验动物管理补助(2008)

摘　　要：人ZNF256基因属于C2H2型锌指蛋白家族。该家族里面的基因大部分为转录因子，它们能够促进细胞分化、参与胚胎及心脏的发育，与精子的形成有关，并且与肿瘤相关。本文采用实时定量PCR方法分析了ZNF256基因在各个组织和不同细胞系中的表达情况，亚细胞定位发现ZNF256定位于细胞核，萤光素酶实验分析确定ZNF256可以抑制AP1的转录活性。这些研究结果为进一步研究ZNF256基因在疾病发生中的作用奠定了一定的基础。The gene ZNF256 belongs to C2H2 Zinc finger protein families in which most of the proteins are transcriptional factors, and they are involved in cell differentiation, embryonic and heart development, sperm formation and cancer etc. In this paper, the coding region of human ZNF256 was cloned, the expression profile of ZNF256 gene was studied in different cell lines and tissues by using real-time PCR. ZNF256 protein was located in the nucleus with laser confocal microscopy. Luciferase assay indicated that ZNF256 could inhibit the transcriptional activity of API, suggesting that ZNF256 was a transcriptional inhibitor. Our results provided a basis for further investigating the function of human ZNF256 in embryo development and the related diseases occurring.

关 键 词：ZNU256 表达谱 荧光实时定量PCR 转录活性 

分 类 号：Q78[生物学—分子生物学]