几种同工酶与猕猴桃品种对溃疡病抗性关系的研究  

A Study on the Relationship between Isozyme and the Resistance of Kiwifruit Cultivars to Bacterial Canker by Pseuomonas syringae pv.actinidae

作　　者：李淼[1] 檀根甲[1] 李瑶[2] 承河元[1] 丁克坚[1] 薛莲[1] 

机构地区：[1]安徽农业大学植物保护学院,安徽合肥230036 [2]安徽农业大学园艺学院,安徽合肥230036

出　　处：《激光生物学报》2009年第2期218-225,共8页Acta Laser Biology Sinica

基　　金：安徽省重点科研项目(02023003);安徽省教育厅自然科学研究项目(95-农-18)

摘　　要：噬加聚内烯酰胺凝胶电泳、同工酶分析技术分别研究了猕猴桃植株怵内过氧化物酶（POD）、多酚氧化酶（PPO）、超氧化物歧化酶（SOD）、过氧化氛酶（CAT）、酯酶（EST）同工酶谱带的变化，结果表明：自然感染溃疡病前后，此6种同工酶潜带特征在不同抗感品种中表现出一定的差芹。未感染溃疡病菌前，抗（感）品系枝条、叶片POD同工酶均有2条酶带，PPO同工酶有3条酶带，但感病品种酶带颜色深H粗，而抗病品种酶带颜色浅且细，叶片酶带颜色深于枝条；SOD、CAT同上酶谱带均为1条，Rf值分别为0．38、0．28，感性品种较抗耐品种谱带亮瞍高活性强；自然发病后，抗（感）品系POD、PPO同工酶谱带数都增加，分别为4、3条和5、4条，且抗病品种新酶带出现较感痫品种早且酶带粗颜色深活性强，感病品系虽也有新酶带出现，但酶带少活性弱，抗病品系枝条、叶片POD、PPO同工酶新谱带的Rf值分别为0．63、0．67和0．85、0．87；抗感病品种SOD、CAT同工酶都被诱导产牛了1条新的同工酶谱带，Rf分别为0．32和0．27，新酶带现色时间迟，且酶带颜色浅活性弱，但抗耐品种较感性品种谱带亮且活性强；EST同上酶于自然发病前后变化小大，与抗病性天系不很明显。The application of polyacrylamide gel eleetrophoresis （PAGE） and isoenzyme analytic technique to study the varialions of isozyme spectra of peroxidase enzyme （POD） , polyphenol oxidase （PPO） , superoxide dismutase （SOD） , catalase （CAT） , and esterase （EST） in kiwifruit plant. The results showed that pre- and post- natural infection by Pseuomonas syringae pv. actinidae, the charaeter of such six isozymes spectra had some differentia in susceptible and resistant cuhivars. Pre-infection by Pseuomonas syringae pv. actinidae in the field, isozyme spectra of POD and PPO had 2 and 3 bands respectively in the annual twig and foliage of both susceptible and resistant cuhivars, but there were also differences with regard to both width and colour, isoenzyme bands of susceptible cultivars were darker in colour and thin in width, and yet isoenzyme bands of resistant cultivars were shallower in colour and thin in width, isoenzyme bands of foliage were darker in colour than those of the annual twig, isozyme spectra of SOD and CAT all had 1 band only, relative mobilities（Rf） numerical value were 0.38, 0.28 respectively, isoenzyme bands of susceptible eultivars were lighter in colout and strong in activity. Post-infection by Pseuomonas syringae pv. actinidae in the field, isozyme bands of both POD and PPO increased, having 4, 3 and 5, 4 respectively, and isozyme bands produced newly in resistant euhivars appeared early in time, wide in width and strong in activity, although isozyme bands produced newly in susceptible cuhivars, yet they appeared late in time, few in number and weak in activity, isozyme bands of POD and PPO produced newly in the annual twig and foliage of resistant cuhivars, and their Rf numerical value were 0.63, 0.67 and 0.85, 0.87 respectively. Isozyme of SOD and CAT were induced and produced 1 band uewly in both resistant and susceptible cultivars, Rf numerical value were 0.32, 0.27 respectively, new isozyme bands appeared late, light in colour and weak in activity, but isozyme band

关 键 词：猕猴桃 溃疡病 同工酶 品种抗性 抗性机理 

分 类 号：S663.4[农业科学—果树学] Q946.5[农业科学—园艺学]